04-1549

Anti-SAP130 Antibody, clone 1YO 1B10

ascites fluid, clone 1YO 1B10, from mouse

• Synonym(s): 130 kDa Sin3-associated polypeptide, Sin3-associated polypeptide p130, Sin3A-associated protein, 130kDa, histone deacetylase complex subunit SAP130, mSin3A-associated protein 130 ; ; Spliceosome-associated protein 130, Pre-mRNA-splicing factor SF3b
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: 1YO 1B10, monoclonal
• species reactivity: mouse, rat, human
• technique(s): immunocytochemistry: suitable; western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_598714.1
• UniProt accession no.: Q921M3
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... SAP130(79595)

Description

General description

SAP130, also known as Splicing factor 3B subunit 3, is part of the RNA splicing machinery. It is expressed by the Sf3b3 gene and encodes subunit 3 of the splicing factor 3b protein complex. As a component of this protein complex, it is associated with U2 snRNP and recruited to prespliceosomal complexes. Splicing factor 3b along with splicing factor 3a and a 12S RNA, form the U2 small nuclear ribonucleoprotein complex (U2 snRNP). In pre-mRNA’s, the splicing factor 3b/3a complex binds upstream of intron branch sites possibly anchoring the U2 snRNP to the pre-mRNA.
SAP130 is also a component of the STAGA (SPT3-TAF(II)31-GCN5L acetylase) transcription coactivator-HAT (histone acetyltransferase) complex, and the TFTC (TATA-binding-protein-free TAF(II)-containing complex). These complexes are believed to function in chromatin modification, transcription, splicing, and DNA repair.

Specificity

This antibody recognizes SAP130.

Immunogen

Ovalbumin-conjugated linear peptide corresponding to mouse SAP130.

Application

Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected SAP130 in NIH/3T3 cells.

Use Anti-SAP130 Antibody, clone 1YO 1B10 (Mouse Monoclonal Antibody) validated in WB, ICC to detect SAP130 also known as 130 kDa Sin3-associated polypeptide, SF3b130.

Quality

Evaluated by Western Blot in RAW264.7 cell lysate.

Western Blot Analysis: 1:1,000 dilution of this antibody detected SAP130 on 10 µg of RAW264.7 cell lysate.

Target description

~ 136 kDa

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable