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Sigma-Aldrich

DLAM-LVproR-13CH3 Methyl Labeling Kit

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1 G
$271.04
5 G
$992.20

$271.04

Estimated to ship onMay 15, 2025

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All Photos(1)

Select a Size

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1 G
$271.04
5 G
$992.20

About This Item

UNSPSC Code:
12352200
NACRES:
NA.12

$271.04

Estimated to ship onMay 15, 2025

Request more information

technique(s)

bio NMR: suitable

Quality Level

200

shipped in

dry ice

storage temp.

−70°C

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4-Pentylbenzoyl chloride 96%

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4-Pentylbenzoyl chloride

6-Chlorohexanoyl chloride 96%

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6-Chlorohexanoyl chloride

Chlorocarbonylsulfenyl chloride 96%

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Chlorocarbonylsulfenyl chloride

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

refractive index

n20/D 1.5560 (lit.)

refractive index

n20/D 1.53 (lit.)

refractive index

n20/D 1.4640 (lit.)

refractive index

n20/D 1.517 (lit.)

density

1.460 g/mL at 25 °C (lit.)

density

1.036 g/mL at 25 °C (lit.)

density

1.400 g/mL at 25 °C (lit.)

density

1.552 g/mL at 25 °C (lit.)

General description

DLAM-LVproS-13CH3 kit has 13CH3 Isotopomer precursors and contains protocol instructions for creation of isotopically-labeled proteins.

Application

For detection of long-range nOes or for the NMR study of large proteins
Methyl groups of leucine and valine is labeled with 13CH3 using DLAM-LVproS-13CH3 kit. This kit has been tested with protein isotopic labeling in E. coli. DLAM-LVproS-13CH3 is used in NMR spectroscopy to provide site-resolved information for intrinsically disordered protein or regions of proteins in NMR spectroscopic studies.

Packaging

This product may be available from bulk stock and can be packaged on demand. For information on pricing, availability and packaging, please contact Stable Isotopes Customer Service.

Storage Class Code

11 - Combustible Solids

  • Specification Sheet

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    Lot/Batch Number
    MBBD1362

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    Stereospecific isotopic labeling of methyl groups for NMR spectroscopic studies of high-molecular-weight proteins.
    Pierre Gans et al.
    Angewandte Chemie (International ed. in English), 49(11), 1958-1962 (2010-02-17)
    Rime Kerfah et al.
    Current opinion in structural biology, 32, 113-122 (2015-04-17)
    Nuclear magnetic resonance (NMR) spectroscopy is a uniquely powerful tool for studying the structure, dynamics and interactions of biomolecules at atomic resolution. In the past 15 years, the development of new isotopic labeling strategies has opened the possibility of exploiting
    Silke Wiesner et al.
    Current opinion in structural biology, 35, 60-67 (2015-09-26)
    Intermolecular interactions are indispensible for biological function. Here we discuss how novel NMR techniques can provide unique insights into the assembly, dynamics and regulation of biomolecular complexes. We focus on applications that exploit the methyl TROSY effect and show that

    Articles

    Methyl Isotope-Labeling of Proteins from NMR-Bio

    Methyl groups are ideal probes for studying protein structure and function by NMR. Choose among user-friendly kits for isotope labeling of a single type or multiple types of methyl groups.

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

    Contact Technical Service