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Merck

N1891

Sigma-Aldrich

p-Nitrophenyl phosphate

SIGMAFAST™, chromogenic, tablet

Synonym(s):

pNPP tablets

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About This Item

MDL number:
MFCD00284586
UNSPSC Code:
12352204
NACRES:
NA.83

Key Documents

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Product Name

SIGMAFAST™ p-Nitrophenyl phosphate Tablets, tablet, To prepare 5 mL

Quality Level

200

form

tablet

solubility

deionized water: soluble 5 mL

storage temp.

−20°C

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Application

Developed for use as a soluble substrate for the detection of alkaline phosphatase activity in Enzyme Immunoassays (EIA). pNPP is the substrate of choice in high sensitivity alkaline phosphatase-based EIA assays.One pNPP tablet and one Tris buffer tablet are dissolved in deionized water and provide a ready-to-use substrate. Each SIGMAFAST pNPP tablet set yields a solution containing 1.0 mg/ml pNPP, 0.2 M Tris buffer. EIA applications using pNPP may be read in timed assays or stopped with 3N NaOH for delayed readings. SIGMAFAST™ require only the addition of distilled or deionized water to prepare an active substrate solution. Tablets are individually packaged in foil packets.
SIGMA FAST p-Nitrophenyl phosphate Tablets has been used:
  • to quantify non-secreted alkaline phosphatase to monitor differentiation of osteoblast-like cells (SaoS-2)
  • to stain murine preosteoblast MC3T3-E1 cells in alkaline phosphatase assay
  • as a substrate for ExtrAvidin® alkaline phosphatase to measure hemoglobin-bound α-synuclein using enzyme-linked immunosorbent assay (ELISA)
  • as a substrate for alkaline phosphatase conjugated antibodies for detection in ELISA of abirin toxin

Reconstitution

Each tablet set dissolved in 5 mL deionized water yields a ready-to-use buffered solution containing pNPP.

Legal Information

ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany
SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

N1891-BULK:
N1891-VAR:
N1891-5SET:
N1891-50SET:

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Certificates of Analysis (COA)

Lot/Batch Number
SLCH7212
SLCB9803
SLBW4265
SLBS5640
SLBN9968V

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