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  • BBC3 in macrophages promoted pulmonary fibrosis development through inducing autophagy during silicosis.

BBC3 in macrophages promoted pulmonary fibrosis development through inducing autophagy during silicosis.

Cell death & disease (2017-03-10)
Haijun Liu, Yusi Cheng, Jian Yang, Wei Wang, Shencun Fang, Wei Zhang, Bing Han, Zewei Zhou, Honghong Yao, Jie Chao, Hong Liao
ABSTRACT

Following inhalation into the lungs, silica particles are engulfed by alveolar macrophages, which triggers endogenous or exogenous apoptosis signaling pathways. As an inducer of apoptosis, the role of BBC3/PUMA (BCL2-binding component 3) in macrophages during silicosis remains unknown. Here, we exposed U937 cell-derived macrophages (UDMs) to SiO

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
PMA, for use in molecular biology applications, ≥99% (HPLC)
Sigma-Aldrich
Silicon dioxide, ~99%, 0.5-10 μm (approx. 80% between 1-5 μm)
Sigma-Aldrich
Anti-SMA antibody, Rabbit monoclonal, clone SP171, recombinant, expressed in proprietary host, affinity isolated antibody