- Substantial Differentiation of Human Neural Stem Cells Into Motor Neurons on a Biomimetic Polyurea.
Substantial Differentiation of Human Neural Stem Cells Into Motor Neurons on a Biomimetic Polyurea.
Macromolecular bioscience (2015-06-03)
Donghwa Yun, Young M Lee, Melissa R Laughter, Curt R Freed, Daewon Park
PMID26033933
ABSTRACT
To find the first restorative treatment for spinal cord injury (SCI), researchers have focused on stem cell therapies. However, one obstacle is the lack of an implantable cell scaffold that can support efficient motor neuron (MN) differentiation and proliferation. We aimed to overcome this through the use of an RGD functionalized novel biomimetic polyurea, optimized to encourage efficient differentiation of MNs. Images taken after 14-days showed increased differentiation (∼40%) of hNSCs into MNs as well as increased cell count on the biomimetic polymer compared to PDL-Laminin coating, indicating that the RGD-polyurea provides a favorable microenvironment for hNSC survival, having promising implications for future SCI therapies.
MATERIALS
Product Number
Brand
Product Description
Sigma-Aldrich
Dichloromethane, suitable for HPLC, ≥99.9%, contains 40-150 ppm amylene as stabilizer
Sigma-Aldrich
Urea, puriss., meets analytical specification of Ph. Eur., BP, USP, 99.0-100.5%, 99.0-101.0% (calc. on dry substance)