Study of the persistence of the anti-inflammatory effect observed after application of preparations containing organic ultraviolet filters.

Study of the persistence of the anti-inflammatory effect observed after application of preparations containing organic ultraviolet filters.

International journal of pharmaceutics (2014-10-01)

C Couteau, C Chauvet, E Paparis, L J M Coiffard

ABSTRACT

Given that previous studies have highlighted the anti-inflammatory nature, which is influenced by UV radiation, of a certain number of ultraviolet filters currently used in Europe, it seemed interesting to evaluate the persistence of this type of effect. The persistence of the anti-inflammatory effect of fourteen preparations, each containing one of the ultraviolet filters authorized by Regulation (CE) No. 1223/2009, and of 10 commercially-available sun products was studied using the phorbol-myristate-acetate test on mice, up to 6 and a half hours after application. We can observe that a benzophenone, oxybenzone, a PABA derivative, octyldimethylPABA and a derivative of cinnamic acid, OMC as well as 3 commercially-available products, display a very marked anti-inflammatory effect at the end of our experimentation phase. The type of effect observed could encourage users to prolong the time that they are exposed to the sun, due to there being no warning signs, namely sunburn.

MATERIALS

Product Number

Brand

Product Description

PHR1074

Supelco

Oxybenzone, Pharmaceutical Secondary Standard; Certified Reference Material

59647

Supelco

Oxybenzone, analytical standard

635057

Sigma-Aldrich

Titanium(IV) oxide, rutile, <001>, (single crystal substrate), ≥99.9% trace metals basis, L × W × thickness 10 mm × 10 mm × 0.5 mm

15524

Sigma-Aldrich

Glycerol solution, puriss., meets analytical specification of Ph. Eur., BP, 84-88%

T2545000

Trolamine, European Pharmacopoeia (EP) Reference Standard

1485001

USP

Oxybenzone, United States Pharmacopeia (USP) Reference Standard

73487

Supelco

Triethylamine, analytical standard

634662

Sigma-Aldrich

Titanium(IV) oxide, mixture of rutile and anatase, nanopowder, <100 nm particle size (BET), 99.5% trace metals basis

637262

Sigma-Aldrich

Titanium(IV) oxide, rutile, nanopowder, <100 nm particle size, 99.5% trace metals basis

T0886

Sigma-Aldrich

Triethylamine, ≥99%

P8139

Sigma-Aldrich

Phorbol 12-myristate 13-acetate, ≥99% (TLC), film or powder

224227

Sigma-Aldrich

Titanium(IV) oxide, rutile, powder, <5 μm, ≥99.9% trace metals basis

79346

Sigma-Aldrich

Phorbol 12-myristate 13-acetate, synthetic, ≥98.0% (TLC)

90335

Sigma-Aldrich

Triethylamine, BioUltra, ≥99.5% (GC)

415820

Sigma-Aldrich

2-Ethylhexyl 2-cyano-3,3-diphenylacrylate, 97%

204757

Sigma-Aldrich

Titanium(IV) oxide, rutile, ≥99.98% trace metals basis

204730

Sigma-Aldrich

Titanium(IV) oxide, rutile, 99.995% trace metals basis

W424601

Sigma-Aldrich

Triethylamine, ≥99.5%

90340

Sigma-Aldrich

Triethylamine, puriss. p.a., ≥99.5% (GC)

90337

Sigma-Aldrich

Triethylamine, for amino acid analysis, ≥99.5% (GC)

49781

Sigma-Aldrich

Glycerol solution, 83.5-89.5% (T)

90338

Sigma-Aldrich

Triethylamine, for protein sequence analysis, ampule, ≥99.5% (GC)

700347

Sigma-Aldrich

Titanium(IV) oxide, mixture of rutile and anatase, nanoparticles, <150 nm particle size (volume distribution, DLS), dispersion, 40 wt. % in H₂O, 99.5% trace metals basis

471283

Sigma-Aldrich

Triethylamine, ≥99.5%

30-4210

Sigma-Aldrich

Triethylamine, SAJ first grade, ≥98.0%

14027

Sigma-Aldrich

Titanium(IV) oxide, puriss., meets analytical specification of Ph. Eur., BP, USP, 99-100.5%

637254

Sigma-Aldrich

Titanium(IV) oxide, anatase, nanopowder, <25 nm particle size, 99.7% trace metals basis