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  • Quantitative determination of 26 steroids in eggs from various species using liquid chromatography-triple quadrupole-mass spectrometry.

Quantitative determination of 26 steroids in eggs from various species using liquid chromatography-triple quadrupole-mass spectrometry.

Journal of chromatography. A (2014-07-16)
Xiaoxia Mi, Sicong Li, Yanhua Li, Kaiqiang Wang, Dan Zhu, Gang Chen
ABSTRACT

A method for analyzing 26 types of steroids in egg matrix was developed. The method used liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) in electrospray ionization mode (ESI). The procedure involved extraction with acetonitrile and removal of phospholipids by zinc chloride (ZnCl₂) followed by SPE cleanup with a Plexa cartridge. The effect of ZnCl₂ on phospholipid removal was directly observed using the post column infusion procedure. The SPE washing and elution conditions were optimized using a shallow gradient procedure. The free and conjugated steroids forms were determined using enzyme hydrolysis. The developed method resulted in satisfactory precision (RSD≤15%), and the limits of quantification were between 0.05 and 25 ng/g depending on the steroid types. The recoveries ranged from 63.2% to 121.5%. Finally, the developed method was successfully applied to compare the steroids in eggs from different species (i.e., hen, duck, quail and pigeon eggs) or different raising system (i.e., normal vs. organic eggs). The steroids can be clearly clustered according to species and raising system. The hierarchical clustering analysis indicated similarity of the steroids among the species. The developed method is sensitive and useful for detection and quantification of steroids in eggs and can be used for residue control programs. In addition, the observed steroid content will provide a fundamental reference for food risk assessment analysis.

MATERIALS
Product Number
Brand
Product Description

USP
Estrone, United States Pharmacopeia (USP) Reference Standard
Supelco
Testosterone solution, 1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®
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Acetic acid, glacial, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.8%
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Acetic acid, glacial, puriss., 99-100%
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Acetic acid, glacial, puriss., meets analytical specification of Ph. Eur., BP, USP, FCC, 99.8-100.5%
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Acetic acid, glacial, ≥99.99% trace metals basis
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Acetic acid, glacial, ReagentPlus®, ≥99%
Methyltestosterone, European Pharmacopoeia (EP) Reference Standard
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Acetic acid, glacial, ACS reagent, ≥99.7%
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trans-Dehydroandrosterone, ≥99%
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17α-Methyltestosterone, solid (photosensitive)
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Testosterone, ≥98%
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Corticosterone, ≥92%
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Progesterone, meets USP testing specifications
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Prednisolone, ≥99%
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Prednisone, ≥98%
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Hydrocortisone, BioReagent, suitable for cell culture
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Progesterone, γ-irradiated, BioXtra, suitable for cell culture
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Hydrocortisone, γ-irradiated, powder, BioXtra, suitable for cell culture
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Hydrocortisone, meets USP testing specifications
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Diethylstilbestrol, VETRANAL®, analytical standard
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17α-Methyltestosterone, VETRANAL®, analytical standard
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Progesterone, VETRANAL®, analytical standard
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Corticosterone, ≥98.5% (HPLC)
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Acetic acid, for luminescence, BioUltra, ≥99.5% (GC)
Supelco
Testosterone, VETRANAL®, analytical standard
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Acetic acid, ≥99.5%, FCC, FG
Supelco
Prednisolone, VETRANAL®, analytical standard
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Acetic acid solution, 1 M, 1 N
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Acetic acid, ≥99.7%