Skip to Content
MilliporeSigma
  • The anoikis effector Bit1 displays tumor suppressive function in lung cancer cells.

The anoikis effector Bit1 displays tumor suppressive function in lung cancer cells.

PloS one (2014-07-09)
Xin Yao, Scott Jennings, Shubha Kale Ireland, Tri Pham, Brandi Temple, Mya Davis, Renwei Chen, Ian Davenport, Hector Biliran
ABSTRACT

The mitochondrial Bit1 (Bcl-2 inhibitor of transcription 1) protein is a part of an apoptotic pathway that is uniquely regulated by integrin-mediated attachment. As an anoikis effector, Bit1 is released into the cytoplasm following loss of cell attachment and induces a caspase-independent form of apoptosis. Considering that anoikis resistance is a critical determinant of transformation, we hypothesized that cancer cells may circumvent the Bit1 apoptotic pathway to attain anchorage-independence and tumorigenic potential. Here, we provide the first evidence of the tumor suppressive effect of Bit1 through a mechanism involving anoikis induction in human lung adenocarcinoma derived A549 cells. Restitution of Bit1 in anoikis resistant A549 cells is sufficient to induce detachment induced-apoptosis despite defect in caspase activation and impairs their anchorage-independent growth. Conversely, stable downregulation of Bit1 in these cells significantly enhances their anoikis resistance and anchorage-independent growth. The Bit1 knockdown cells exhibit significantly enhanced tumorigenecity in vivo. It has been previously shown that the nuclear TLE1 corepressor is a putative oncogene in lung cancer, and we show here that TLE1 blocks Bit1 mediated anoikis in part by sequestering the pro-apoptotic partner of Bit1, the Amino-terminal Enhancer of Split (AES) protein, in the nucleus. Taken together, these findings suggest a tumor suppressive role of the caspase-independent anoikis effector Bit1 in lung cancer. Consistent with its role as a tumor suppressor, we have found that Bit1 is downregulated in human non-small cell lung cancer (NSCLC) tissues.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
MISSION® esiRNA, targeting human PTRH2
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Dimethyl sulfoxide, ≥99.0%, suitable for absorption spectrum analysis
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Dimethyl sulfoxide, ≥99.5%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Dimethyl sulfoxide solution, 50 wt. % in H2O
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Dimethyl sulfoxide, JIS special grade, ≥99.0%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Dimethyl sulfoxide, SAJ first grade, ≥99.0%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Dimethyl sulfoxide, suitable for HPLC
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
MISSION® esiRNA, targeting human TLE1
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
MISSION® esiRNA, targeting mouse Ptrh2
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Dimethyl sulfoxide, meets EP testing specifications, meets USP testing specifications
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Dimethyl sulfoxide, ≥99.5% (GC), suitable for plant cell culture
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Dimethyl sulfoxide, Hybri-Max, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Dimethyl sulfoxide, anhydrous, ≥99.9%
SKU
Pack Size
Availability
Price
Quantity
USP
Dimethyl sulfoxide, United States Pharmacopeia (USP) Reference Standard
SKU
Pack Size
Availability
Price
Quantity
Dimethyl sulfoxide, European Pharmacopoeia (EP) Reference Standard
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Dimethyl sulfoxide, sterile-filtered, BioPerformance Certified, meets EP, USP testing specifications, suitable for hybridoma
SKU
Pack Size
Availability
Price
Quantity
Supelco
Dimethyl sulfoxide, analytical standard
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
8-Octanoyloxypyrene-1,3,6-trisulfonic acid trisodium salt, suitable for fluorescence, ≥90% (HPCE)
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Dimethyl sulfoxide, suitable for HPLC, ≥99.7%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Dimethyl sulfoxide, ReagentPlus®, ≥99.5%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Dimethyl sulfoxide, ACS reagent, ≥99.9%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Dimethyl sulfoxide, puriss. p.a., dried, ≤0.02% water
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Dimethyl sulfoxide, for molecular biology
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Dimethyl sulfoxide, PCR Reagent
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Dimethyl sulfoxide, Vetec, reagent grade, 99%
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Dimethyl sulfoxide, puriss. p.a., ACS reagent, ≥99.9% (GC)
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Dimethyl sulfoxide, BioUltra, for molecular biology, ≥99.5% (GC)
SKU
Pack Size
Availability
Price
Quantity
Supelco
Dimethyl sulfoxide, for inorganic trace analysis, ≥99.99995% (metals basis)
SKU
Pack Size
Availability
Price
Quantity