Skip to Content
MilliporeSigma
  • Peripheral blood monocyte-derived chemokine blockade prevents murine transfusion-related acute lung injury (TRALI).

Peripheral blood monocyte-derived chemokine blockade prevents murine transfusion-related acute lung injury (TRALI).

Blood (2014-03-19)
Christopher G J McKenzie, Michael Kim, Tarandeep K Singh, Youli Milev, John Freedman, John W Semple
ABSTRACT

Transfusion-related acute lung injury (TRALI) is the leading cause of transfusion-related mortality and can occur with any type of transfusion. TRALI is thought to be primarily mediated by donor antibodies activating recipient neutrophils resulting in pulmonary endothelial damage. Nonetheless, details regarding the interactions between donor antibodies and recipient factors are unknown. A murine antibody-mediated TRALI model was used to elucidate the roles of the F(ab')2 and Fc regions of a TRALI-inducing immunoglobulin G anti-major histocompatibility complex (MHC) class I antibody (34.1.2s). Compared with intact antibody, F(ab')2 fragments significantly increased serum levels of the neutrophil chemoattractant macrophage inflammatory protein 2 (MIP-2); however, pulmonary neutrophil levels were only moderately increased, and no pulmonary edema or mortality occurred. Fc fragments did not modulate any of these parameters. TRALI induction by intact antibody was completely abrogated by in vivo peripheral blood monocyte depletion by gadolinium chloride (GdCl3) or chemokine blockade with a MIP-2 receptor antagonist but was restored upon repletion with purified monocytes. The results suggest a two-step process for antibody-mediated TRALI induction: the first step involves antibody binding its cognate antigen on blood monocytes, which generates MIP-2 chemokine production that is correlated with pulmonary neutrophil recruitment; the second step occurs when antibody-coated monocytes increase Fc-dependent lung damage.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Mouse MIP-2 / CXCL2 ELISA Kit, for serum, plasma and cell culture supernatant
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Fluorescein isothiocyanate isomer I, ≥97.5% (HPLC)
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Fluorescein isothiocyanate isomer I, ≥97.5% (HPLC)
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Fluorescein isothiocyanate isomer I, suitable for protein labeling, ≥90% (HPLC), powder
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Human IgG ELISA Kit, for serum, plasma
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Rat CINC-3 ELISA Kit, for serum, plasma and cell culture supernatant
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Mouse IgG3 ELISA Kit
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Mouse IgG2a ELISA Kit
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Fluorescein 5(6)-isothiocyanate, ≥90% (HPLC)
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Hematoxylin, certified by the Biological Stain Commission
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Hematoxylin
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Fluorescein 5(6)-isothiocyanate, BioReagent, suitable for fluorescence, mixture of 2 components, ≥90% (HPLC)
SKU
Pack Size
Availability
Price
Quantity
Supelco
Gadolinium(III) chloride solution, NMR reference standard, water 1 %, DSS 0.1 %, 0.1 mg/mL in D2O (99.9 atom % D), NMR tube size 5 mm × 8 in.
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Gadolinium(III) chloride, anhydrous, powder, 99.99% trace metals basis
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Gadolinium(III) chloride solution, NMR reference standard, 0.1 mg/mL in D2O (99.9 atom % D), water 0.1 %, NMR tube size 6.5 mm × 8 in.
SKU
Pack Size
Availability
Price
Quantity
Sigma-Aldrich
Gadolinium(III) chloride solution, NMR reference standard, 0.1 mg/mL in D2O (99.9 atom % D), Methanol-13C 0.1 % (99 atom % 13C), water 1 %, NMR tube size 3 mm × 8 in.
SKU
Pack Size
Availability
Price
Quantity