Skip to Content
MilliporeSigma
  • Determination of chlorobenzenes in water samples by solid-phase disk extraction and gas chromatography-electron capture detection.

Determination of chlorobenzenes in water samples by solid-phase disk extraction and gas chromatography-electron capture detection.

Journal of chromatographic science (2013-05-07)
Hongmei Hu, Yuanming Guo, Xiumei Sun, Xuechang Chen, Xiaoning Zhang, Qin Liu, Chunxiu Xu
ABSTRACT

A simple, rapid, sensitive and high throughput method is described, based on solid-phase disk extraction (SPDE) and gas chromatography-electron capture detection, for the determination of chlorobenzens (CBs) in water samples. The proposed SPDE sample pretreatment method was initially optimized and the optimum experimental conditions were found to be as follows: 500 mL water sample (pH 2.5) extracted and enriched by an Empore 3-stn C18 (octadecyl) SPE disk at flow rate of 5 to 50 mL/min, eluted by 5 mL of acetone and 3 × 5 mL of methylene dichloride. The linearity of the method ranged from 0.02 to 0.4 µg/L for dichlorobenzene isomers, 0.0022-0.044 µg/L for trichlorobenzene isomers, 0.005-0.01 µg/L for tetrachlorobenzene isomers and 0.00025 to 0.005 µg/L for pentachlorobenzenes and hexachlorobenzenes, with correlation coefficients ranging between 0.9991 and 0.9999. The limits of detection were in the low ng/L level, ranging between 0.05 and 4 ng/L. The recoveries of spiked CBs with the external calibration method at different concentration levels in deionized/distilled water, tap water and sea water samples were 99-115, 91-106% and 96-110%, respectively, and with relative standard deviations of 4.5-7.6, 4.2-6.8 and 3.6-6.6% (n = 5), respectively. It is concluded that this method can successfully be applied for the determination of CBs in deionized/distilled water, tap water and sea water samples.

MATERIALS
Product Number
Brand
Product Description

Supelco
1,3,5-Trichlorobenzene, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland
Sigma-Aldrich
Methanol, NMR reference standard
Sigma-Aldrich
Methanol, SAJ special grade
Sigma-Aldrich
Methanol, HPLC Plus, ≥99.9%, poly-coated bottles
SAFC
Sodium chloride solution, 5 M
Sigma-Aldrich
Sodium chloride, BioUltra, for molecular biology, ≥99.5% (AT)
Sigma-Aldrich
Methanol, anhydrous, 99.8%
Sigma-Aldrich
1,3,5-Trichlorobenzene, 99%
Sigma-Aldrich
Methanol, suitable for HPLC, gradient grade, 99.93%
Sigma-Aldrich
Sodium chloride-35Cl, 99 atom % 35Cl
Sigma-Aldrich
Sodium chloride, random crystals, optical grade, 99.9% trace metals basis
Sigma-Aldrich
Methanol, JIS 300, ≥99.8%, for residue analysis
Sigma-Aldrich
Methanol, JIS special grade, ≥99.8%
Sigma-Aldrich
Chlorobenzene, SAJ special grade, ≥99.5%
Sigma-Aldrich
Sodium chloride, SAJ first grade, ≥99.0%
Sigma-Aldrich
Chlorobenzene, SAJ first grade, ≥99.0%
Sigma-Aldrich
Methanol, SAJ first grade, ≥99.5%
Sigma-Aldrich
Sodium chloride, JIS special grade, ≥99.5%
Sigma-Aldrich
Methanol, Laboratory Reagent, ≥99.6%
Sigma-Aldrich
Chlorobenzene, anhydrous, 99.8%
Sigma-Aldrich
Methanol, suitable for HPLC, ≥99.9%
Sigma-Aldrich
Methanol, HPLC Plus, ≥99.9%
Sigma-Aldrich
Methanol, Absolute - Acetone free
Sigma-Aldrich
Sodium chloride, BioPerformance Certified, ≥99% (titration), suitable for insect cell culture, suitable for plant cell culture
Sigma-Aldrich
1,2,4-Trichlorobenzene, anhydrous, ≥99%
Supelco
1,2,4-Trichlorobenzene, PESTANAL®, analytical standard
Sigma-Aldrich
1,2,3-Trichlorobenzene, 99%
Supelco
Methanol, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
Methanol, ACS spectrophotometric grade, ≥99.9%
Supelco
Methanol, analytical standard