- Characterization of a cDNA from Beta maritima that confers nickel tolerance in yeast.
Characterization of a cDNA from Beta maritima that confers nickel tolerance in yeast.
Gene (2014-02-04)
Gonensin O Bozdag, Alaattin Kaya, Ahmet Koc, Gundula A Noll, Dirk Prüfer, Hüseyin Caglar Karakaya
PMID24487090
ABSTRACT
Nickel is an essential micronutrient due to its involvement in many enzymatic reactions as a cofactor. However, excess of this element is toxic to biological systems. Here, we constructed a cDNA library from Beta maritima and screened it in the yeast system to identify genes that confer resistance to toxic levels of nickel. A cDNA clone (NIC6), which encodes for a putative membrane protein with unknown function, was found to help yeast cells to tolerate toxic levels of nickel. A GFP fused form of Nic6 protein was localized to multivesicular structures in tobacco epidermal cells. Thus, our results suggest a possible role of Nic6 in nickel and intracellular ion homeostasis.
MATERIALS
Product Number
Brand
Product Description
Nickel, foil, 0.5m coil, thickness 1.0mm, annealed, 99%
Nickel, foil, 10mm disks, thickness 0.025mm, as rolled, 99.99+%
Nickel, wire reel, 100m, diameter 0.5mm, as drawn, 99%
Nickel, foil, 0.5m coil, thickness 0.0125mm, annealed, 99%
Nickel, wire reel, 0.5m, diameter 0.05mm, as drawn, 99.99+%
Nickel, foil, 2m coil, thickness 0.25mm, annealed, 99.98%
Nickel, tube, 500mm, outside diameter 3.0mm, inside diameter 2.4mm, wall thickness 0.3mm, hard, 99.5%
Nickel, foil, 10mm disks, thickness 0.05mm, 99.9%
Nickel, tube, 500mm, outside diameter 2.5mm, inside diameter 2.25mm, wall thickness 0.125mm, hard, 99.5%
Nickel, rod, 1000mm, diameter 12.7mm, 99.98%
Nickel, foil, 25x25mm, thickness 0.25mm, annealed, 99%
Nickel, wire reel, 10m, diameter 2.0mm, hard, 99.98%
Nickel, foil, 4mm disks, thickness 0.025mm, as rolled, 100%
Nickel, foil, 0.5m coil, thickness 0.025mm, coil width 600mm, as rolled, 99%
Nickel, mesh, 130x130mm, nominal aperture 0.34mm, thickness 0.025mm, wire diameter 0.041mm, 70 wires/inch, open area 80%, electro-formed mesh, 99.9%
Nickel, wire reel, 0.5m, diameter 1.0mm, as drawn, 99.99+%
Nickel, mesh, 30x30mm, nominal aperture 0.43mm, thickness 0.004mm, wire diameter 0.067mm, 51 wires/inch, open area 75%, electro-formed mesh, 99.9%
Nickel, wire reel, 100m, diameter 0.125mm, annealed, 99.98%
Nickel, foil, 0.3m coil, thickness 0.125mm, as rolled, 99.99+%
Nickel, tube, 5mm, outside diameter 0.8mm, inside diameter 0.5mm, wall thickness 0.15mm, hard, 99.5%
Nickel, tube, 500mm, outside diameter 1.0mm, inside diameter 0.8mm, wall thickness 0.1mm, hard, 99.5%
Nickel, foil, 10m coil, thickness 0.125mm, coil width 50mm, annealed, 99%
Nickel, pellets, 20g, max. size 20mm, 99.99+%
Nickel, foil, 0.5m coil, thickness 0.125mm, annealed, 99%
Nickel, foil, 0.5m coil, thickness 0.005mm, as rolled, 99.9%
Nickel, foil, 10mm disks, thickness 0.005mm, as rolled, 99.9%
Nickel, rod, 1000mm, diameter 2.0mm, 99.99+%
Nickel, foil, 2m coil, thickness 0.5mm, annealed, 99.98%
Nickel, foil, 2m coil, thickness 0.125mm, annealed, 99.98%
Nickel, foil, 50x50mm, thickness 0.25mm, as rolled, 99.99+%