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  • Characterization of a cDNA from Beta maritima that confers nickel tolerance in yeast.

Characterization of a cDNA from Beta maritima that confers nickel tolerance in yeast.

Gene (2014-02-04)
Gonensin O Bozdag, Alaattin Kaya, Ahmet Koc, Gundula A Noll, Dirk Prüfer, Hüseyin Caglar Karakaya
ABSTRACT

Nickel is an essential micronutrient due to its involvement in many enzymatic reactions as a cofactor. However, excess of this element is toxic to biological systems. Here, we constructed a cDNA library from Beta maritima and screened it in the yeast system to identify genes that confer resistance to toxic levels of nickel. A cDNA clone (NIC6), which encodes for a putative membrane protein with unknown function, was found to help yeast cells to tolerate toxic levels of nickel. A GFP fused form of Nic6 protein was localized to multivesicular structures in tobacco epidermal cells. Thus, our results suggest a possible role of Nic6 in nickel and intracellular ion homeostasis.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Nickel, wire, diam. 0.5 mm, ≥99.99% trace metals basis
Sigma-Aldrich
Nickel, powder, <150 μm, 99.99% trace metals basis
Sigma-Aldrich
Raney®-Nickel, W.R. Grace and Co. Raney® 2800, slurry, in H2O, active catalyst
Sigma-Aldrich
Raney®-Nickel, W.R. Grace and Co. Raney® 2400, slurry, in H2O, active catalyst
Nickel, tube, 200mm, outside diameter 0.8mm, inside diameter 0.3mm, wall thickness 0.25mm, as drawn, 99.5%
Nickel, wire reel, 50m, diameter 0.25mm, as drawn, 99%
Nickel, wire reel, 250m, diameter 0.125mm, as drawn, 99%
Nickel, tube, 200mm, outside diameter 1.3mm, inside diameter 1.1mm, wall thickness 0.1mm, hard, 99.5%
Nickel, foil, 25mm disks, thickness 0.05mm, as rolled, 99.98%
Nickel, foil, 1m coil, thickness 0.03mm, 99.9%
Nickel, tube, 1000mm, outside diameter 3.25mm, inside diameter 3.05mm, wall thickness 0.1mm, hard, 99.5%
Nickel, tube, 1000mm, outside diameter 0.762mm, inside diameter 0.204mm, wall thickness 0.279mm, hard, 99.5%
Nickel, foil, 15mm disks, thickness 0.5mm, annealed, 99.98%
Nickel, tube, 200mm, outside diameter 2.0mm, inside diameter 1.6mm, wall thickness 0.2mm, hard, 99.5%
Nickel, wire reel, 5m, diameter 0.025mm, as drawn, 99.6+%
Nickel, wire reel, 500m, diameter 0.4mm, as drawn, 99%
Nickel, wire reel, 20m, diameter 0.125mm, hard, 99.98%
Nickel, tube, 500mm, outside diameter 0.8mm, inside diameter 0.3mm, wall thickness 0.25mm, as drawn, 99.5%
Nickel, tube, 1000mm, outside diameter 1.5mm, inside diameter 1.4mm, wall thickness 0.05mm, hard, 99.5%
Nickel, foil, 1m coil, thickness 0.0125mm, 99.9%
Nickel, wire reel, 1m, diameter 1.0mm, hard, 99.98%
Nickel, tube, 200mm, outside diameter 0.8mm, inside diameter 0.5mm, wall thickness 0.15mm, as drawn, 99.5%
Nickel, foil, 25mm disks, thickness 0.03mm, 99.9%
Nickel, foil, 10m coil, thickness 0.3mm, coil width 1mm, annealed, 99%
Nickel, foil, 150x150mm, thickness 0.25mm, annealed, 99.98%
Nickel, wire reel, 100m, diameter 0.5mm, annealed, 99%
Nickel, foil, 4mm disks, thickness 0.0125mm, 99.99+%
Nickel, wire reel, 0.2m, diameter 1.0mm, as drawn, 99.99+%
Nickel, foil, 0.5m coil, thickness 0.25mm, annealed, 99%
Nickel, wire reel, 1m, diameter 0.5mm, as drawn, 99.99+%