Characterization of a cDNA from Beta maritima that confers nickel tolerance in yeast.

Characterization of a cDNA from Beta maritima that confers nickel tolerance in yeast.

Gene (2014-02-04)

Gonensin O Bozdag, Alaattin Kaya, Ahmet Koc, Gundula A Noll, Dirk Prüfer, Hüseyin Caglar Karakaya

ABSTRACT

Nickel is an essential micronutrient due to its involvement in many enzymatic reactions as a cofactor. However, excess of this element is toxic to biological systems. Here, we constructed a cDNA library from Beta maritima and screened it in the yeast system to identify genes that confer resistance to toxic levels of nickel. A cDNA clone (NIC6), which encodes for a putative membrane protein with unknown function, was found to help yeast cells to tolerate toxic levels of nickel. A GFP fused form of Nic6 protein was localized to multivesicular structures in tobacco epidermal cells. Thus, our results suggest a possible role of Nic6 in nickel and intracellular ion homeostasis.

MATERIALS

Product Number

Brand

Product Description

267074

Sigma-Aldrich

Nickel, rod, diam. 6.35 mm, ≥99.99% trace metals basis

357553

Sigma-Aldrich

Nickel, foil, thickness 0.5 mm, 99.98% trace metals basis

357626

Sigma-Aldrich

Nickel, wire, diam. 0.5 mm, ≥99.9% trace metals basis

266981

Sigma-Aldrich

Nickel, powder, <50 μm, 99.7% trace metals basis

577995

Sigma-Aldrich

Nickel, nanopowder, <100 nm avg. part. size, ≥99% trace metals basis

357588

Sigma-Aldrich

Nickel, foil, thickness 0.1 mm, 99.98% trace metals basis

GF19402062

Nickel, wire reel, 10m, diameter 0.25mm, annealed, 99%

GF55000628

Nickel, foil, 0.5m coil, thickness 0.075mm, as rolled, 99.98%

GF73711100

Nickel, foil, 4mm disks, thickness 0.007mm, 99.95%

GF09121889

Nickel, foil, 0.5m coil, thickness 0.004mm, hard, 99.98%

GF39653310

Nickel, wire reel, 0.5m, diameter 0.05mm, as drawn, 99.99+%

GF54523111

Nickel, foil, 2m coil, thickness 0.25mm, annealed, 99.98%

GF07087439

Nickel, tube, 500mm, outside diameter 3.0mm, inside diameter 2.4mm, wall thickness 0.3mm, hard, 99.5%

GF64926153

Nickel, foil, 10mm disks, thickness 0.05mm, 99.9%

GF46748144

Nickel, wire reel, 10m, diameter 2.0mm, hard, 99.98%

GF53350163

Nickel, foil, 4mm disks, thickness 0.025mm, as rolled, 100%

GF54340219

Nickel, foil, 0.5m coil, thickness 0.025mm, coil width 600mm, as rolled, 99%

GF49268526

Nickel, tube, 5mm, outside diameter 0.8mm, inside diameter 0.5mm, wall thickness 0.15mm, hard, 99.5%

GF64774345

Nickel, foil, 10m coil, thickness 0.125mm, coil width 50mm, annealed, 99%

GF85861042

Nickel, foil, 0.5m coil, thickness 0.125mm, annealed, 99%

GF98554078

Nickel, tube, 500mm, outside diameter 3.25mm, inside diameter 3.05mm, wall thickness 0.1mm, hard, 99.5%

GF05706389

Nickel, foil, 10mm disks, thickness 0.05mm, annealed, 99%

GF03085321

Nickel, foil, 10mm disks, thickness 0.005mm, as rolled, 99.9%

GF16803075

Nickel, foil, 2m coil, thickness 0.125mm, annealed, 99.98%

GF74649695

Nickel, foil, 2m coil, thickness 0.3mm, annealed, 99%

GF51323093

Nickel, tube, 500mm, outside diameter 3.6mm, inside diameter 3.5mm, wall thickness 0.05mm, hard, 99.5%

GF02127068

Nickel, foil, 10mm disks, thickness 0.0075mm, 99.9%

GF66058612

Nickel, foil, 10mm disks, thickness 0.025mm, 99.9%

GF35355315

Nickel, wire reel, 0.1m, diameter 0.75mm, as drawn, 99.99+%

GF03677665

Nickel, foil, 2m coil, thickness 0.15mm, annealed, 99%