recombinant
expressed in E. coli
Quality Level
conjugate
(N-linked)
form
buffered aqueous solution
specific activity
≥16 U/mg
mol wt
32 kDa
shipped in
wet ice
storage temp.
2-8°C
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General description
Endoglycosidase F1 from Elizabethkingia miricola is a glycan specific enzyme.
Application
Cleaves asparagine-linked or free oligomannose and hybrid, but not complex, oligosaccharides.
Endoglycosidase F1 from Elizabethkingia miricola has been used to remove binding of human hemochromatosis protein (HFE) to cation independent mannose-6-phosphate receptor (CI-MPR) and to glycosylate fucosylated N-glycopeptides.
Biochem/physiol Actions
Endoglycosidase F1 from Elizabethkingia miricola plays an important role in cleavage of glycan structures from the protein by cleaving between the two N-acetylglucosamine residues of the chitobiose core. It mediates high mannose and hybrid oligosaccharides cleavage.
Packaging
Supplied with 5× Reaction Buffer, 250 mM NaH2PO4, pH 5.5.
Unit Definition
One unit will release N-linked oligosaccharides from 1 μmole of denatured Ribonuclease B in 1 minute at 37 °C, pH 5.5.
Physical form
Aseptically filled solution in 20 mM Tris-HCl pH 7.5
Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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