HT 1080

85111505, human fibrosarcoma, Epithelial

• Synonym(s): HT1080 Cells
• UNSPSC Code: 41106514

Properties

• Product Name: HT 1080, 85111505, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 85111505, human fibrosarcoma, Epithelial
• biological source: human fibrosarcoma
• packaging: tube of 5 μg 85111505-DNA-5UG; pkg of vial of cells 85111505-1VL
• growth mode: Adherent
• karyotype: 2n = 46, pseudodiploid
• morphology: Epithelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer

Description

Cell Line Origin

Human fibrosarcoma

Cell Line Description

Established from a fibrosarcoma arising adjacent to the acetabulum of a 35 year old caucasian male. The cells can give rise to tumours in immunosupressed NIH Swiss mice. Susceptible to infection by RNA tumour viruses including RD114 feline endogenous virus and FeLV.

Application

HT 1080 has been used to demonstrate that type I collagen induces matrix metallopeptidase 2 (MMP-2) activation. It has also been used to study the roles of Ras-related C3 botulinum toxin substrate 1 and 2 (Rac1 and Rac2) in regulating morphology, F-actin organization, adhesion, migration and chemotaxis of HT1080 cells.

DNA Profile

STR-PCR Data: Amelogenin: X,Y,
CSF1PO: 12
D13S317: 12,14
D16S539: 9,12
D5S818: 11,13
D7S820: 9,10
THO1: 6
TPOX: 8
vWA: 14,19

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:8 i.e. seeding at 1-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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