IgG, a monomer, is an antibody circulating in blood and can assist in phagocytic destruction of foreign microorganism. It plays an important role in Antibody dependent cell mediated cytotoxicity and is also associated with type II and type III hypersensitivity. Anti-rabbit IgG (whole molecule) peroxidase conjugate antibody can be used in pAb-ELISA. Rabbit anti-IgG antibody coupled with peroxidase (1:2500 dilution in TBS, 0.5% BSA) can be used in western blots. This antibody is also used as detector in indirect ELISA for saxitoxin analysis. Goat anti-rabbit IgG Peroxidase conjugate reacted specifically with all rabbit immunoglobulin.

Purified rabbit IgG.

Anti-rabbit IgG (whole molecule) peroxidase conjugate antibody is used in western blotting, Indirect ELISA, Immunoblotting and Immunodetection.

Co-immunoprecipation and western blot analysis of C33A cell lysates were performed using HRP conjugated goat anti-rabbit IgG as the secondary antibody.

Immunohistochemistry was performed on frozen sections (10um) of mouse intestine, liver, and spleen using HRP-conjugated goat anti-rabbit IgG as the secondary antibody. Prior to incubation with the secondary, sections were treated with a mixture of MeOH/hydrogen peroxide 30% to block endogenouse peroxidases.

Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin with preservative.

Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).

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