A2512
Albumin from chicken egg white
lyophilized powder, ≥98% (agarose gel electrophoresis)
Synonym(s):
Albumin from hen egg white, Ovalbumin
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About This Item
CAS Number:
EC Number:
MDL number:
UNSPSC Code:
12352202
eCl@ss:
42010201
NACRES:
NA.61
Recommended Products
biological source
chicken egg white
Quality Level
type
Grade VI
assay
≥98% (agarose gel electrophoresis)
form
lyophilized powder
mol wt
44287 Da by calculation
purified by
chromatography
concentration
4—20 mol/mol albumin
extent of labeling
4-20 mol mannose per mol ovalbumin protein
technique(s)
bioconjugation: suitable
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General description
Chicken egg albumin is the major protein constituent of egg whites. Chicken egg albumin is a phosphorylated-glycoprotein. From the amino acid sequence, the peptide portion of the molecule consists of 385 residues and has a molecular weight of 42.7 kDa.
Ovalbumin makes 75% of the total egg white protein.
Application
A phosphorylated glycoprotein which is useful as a marker (~45 kDa) for protein electrophoresis. Has been used to produce an animal model of asthma.
Ovalbumin has been used:
- as a medium for cell incubation in EliCell assay for the detection of released cytokines from eosinophil
- to induce allergic inflammation and atopic dermatitis in experimental mice
- as a component in control mixture for validating tandem mass spectral analysis
- as a model protein antigen to evaluate the in vivo efficacy of a chitosan/interleukin (IL)-12 adjuvant system
Biochem/physiol Actions
Albumin from chicken egg white serves as a natural adhesive.
Differential scanning calorimetry indicates that chicken egg albumin denatures at 84 °C. Ovalbumin can be used as a carrier protein to conjugate to synthetic peptides for use as an immunogen. It has the following amino acids: 20 Lys, 10 Tyr, 6 Cys, 14 Asp, and 33 Glu which make it suitable for conjugation.
Physical form
Essentially salt free
Preparation Note
A further purification of A 5503 to reduce mannose content.
signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1A
Storage Class
11 - Combustible Solids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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