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69890

Sigma-Aldrich

MES hydrate

BioUltra, ≥99.5% (T)

Synonym(s):

2-(N-Morpholino)ethanesulfonic acid hydrate, 4-Morpholineethanesulfonic acid

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About This Item

Empirical Formula (Hill Notation):
C6H13NO4S · xH2O
CAS Number:
1266615-59-1
Molecular Weight:
195.24 (anhydrous basis)
MDL number:
MFCD00149409
UNSPSC Code:
12161700
eCl@ss:
32129211
PubChem Substance ID:
57652220
NACRES:
NA.25
Pricing and availability is not currently available.

product line

BioUltra

Quality Level

200

assay

≥99.5% (T)

form

powder or crystals

impurities

insoluble matter, passes filter test

ign. residue (900 °C)

≤0.05%

pH

2.5-4.0 (25 °C, 0.5 M in H2O)

useful pH range

5.5-6.7

pKa 

6.1

solubility

H2O: 0.5 M at 20 °C, clear, colorless

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MES hydrate BioUltra, ≥99.5% (T)

69890

MES hydrate

MES hydrate BioPerformance Certified, suitable for cell culture, ≥99.5%

M2933

MES hydrate

MES hydrate ≥99.5% (titration), pH 2.5-4.0 (0.5 M in H2O), BioXtra

M5287

MES hydrate

MES hydrate free-flowing, Redi-Dri™, ≥99.0%

RDD030

MES hydrate

product line

BioUltra

product line

-

product line

BioXtra

product line

Redi-Dri™

Quality Level

200

Quality Level

400

Quality Level

400

Quality Level

100

form

powder or crystals

form

crystalline powder

form

crystalline powder

form

-

solubility

H2O: 0.5 M at 20 °C, clear, colorless

solubility

water: 335.3 g/L at 20 °C

solubility

H2O: 0.5 M, clear, colorless, water: 335.3 g/L at 20 °C

solubility

-

pH

2.5-4.0 (25 °C, 0.5 M in H2O)

pH

-

pH

2.5-4.0 (0.5 M in H2O)

pH

-

General description

MES is one of the "Good" buffers developed for biological applications. MES is not recommended at pH 7.4; other buffers should be considered.[1] All qualities are tested for trace ions and all can be used for biological and biochemistry applications. This product is used in biochemical and biological techniques where highly purified products are needed.

Preparation Note

A buffer using MES free acid can be prepared by titrating the free acid with sodium hydroxide to the desired pH (pKa=±1). Alternatively, volumes of equimolar MES free acid and sodium MES can be mixed to attain the desired pH. Standard mixing tables using stock solutions to prepare a buffer of a given pH have been published.[2]

Other Notes

Solubility/Stability: MES is soluble in water, giving a clear colourless solution at concentrations of 0.5M or higher. The pH of a solution should be between 2.5 and 5, depending on the concentration. Solutions are stable at 2-8°C for months.
Sterilization: Sterilization should be by filteration through 0.2μM filters. Autoclaving is not recommended by any sulfonic acid buffers. If buffers must be nuclease-free, it is best to treat the water, then add the buffer solids after autoclaving. When MES solutions are autoclaved, they turn yellow (although pH does not change measurably. The identity of the yellow breakdown product is unknown.
Easily compare specifications for MES hydrate products with the MES hydrate specification table.
Biological buffer; useful pH-range 5.8-6.5[3]; Mobile phase component in brain tubulin isolation by amino-activated gel chromatography[4]; Buffer in plant tissue culture[5][6].

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

  • Specification Sheet

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    Certificates of Analysis (COA)

    Lot/Batch Number
    BCCF2829
    BCCC9123
    BCBZ8588
    BCBX4598
    BCBW2580

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    M De Block
    Plant physiology, 93(3), 1110-1116 (1990-07-01)
    Tissue culture conditions and transformation have been established for both aspen and poplar. The use of previously described culture conditions resulted in shoot tip necrosis in the shoot cultures and necrosis of stem and leaf explants. Shoot tip necrosis could
    E Lacey et al.
    Journal of chromatography, 525(1), 71-84 (1990-01-26)
    In the present study, we report the isolation of the acidic structural protein tubulin using a number of amino-activated gels. Crude 100,000 g supernatant derived from sheep brain was applied to gels activated with either aminohexyl, aminoethyl, argininyl, diethylaminoethyl, lysinyl
    D.E. Parfitt
    Sci. Hortic., 36, 157-157 (1988)
    Dawson, R.M.C. et al.
    Data for Biochemical Research, 410- 410 (1987)
    Good NE et al.
    Biochemistry, 5, 467-477 (1966)
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