69890
MES hydrate
BioUltra, ≥99.5% (T)
Synonym(s):
2-(N-Morpholino)ethanesulfonic acid hydrate, 4-Morpholineethanesulfonic acid
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About This Item
Empirical Formula (Hill Notation):
C6H13NO4S · xH2O
CAS Number:
Molecular Weight:
195.24 (anhydrous basis)
MDL number:
UNSPSC Code:
12161700
eCl@ss:
32129211
PubChem Substance ID:
NACRES:
NA.25
Recommended Products
product line
BioUltra
Quality Level
assay
≥99.5% (T)
form
powder or crystals
impurities
insoluble matter, passes filter test
ign. residue (900°C)
≤0.05%
pH
2.5-4.0 (25 °C, 0.5 M in H2O)
useful pH range
5.5-6.7
pKa
6.1
solubility
H2O: 0.5 M at 20 °C, clear, colorless
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General description
MES is one of the "Good" buffers developed for biological applications. MES is not recommended at pH 7.4; other buffers should be considered. All qualities are tested for trace ions and all can be used for biological and biochemistry applications. This product is used in biochemical and biological techniques where highly purified products are needed.
Preparation Note
A buffer using MES free acid can be prepared by titrating the free acid with sodium hydroxide to the desired pH (pKa=±1). Alternatively, volumes of equimolar MES free acid and sodium MES can be mixed to attain the desired pH. Standard mixing tables using stock solutions to prepare a buffer of a given pH have been published.
Other Notes
Solubility/Stability: MES is soluble in water, giving a clear colourless solution at concentrations of 0.5M or higher. The pH of a solution should be between 2.5 and 5, depending on the concentration. Solutions are stable at 2-8°C for months.
Sterilization: Sterilization should be by filteration through 0.2μM filters. Autoclaving is not recommended by any sulfonic acid buffers. If buffers must be nuclease-free, it is best to treat the water, then add the buffer solids after autoclaving. When MES solutions are autoclaved, they turn yellow (although pH does not change measurably. The identity of the yellow breakdown product is unknown.
Sterilization: Sterilization should be by filteration through 0.2μM filters. Autoclaving is not recommended by any sulfonic acid buffers. If buffers must be nuclease-free, it is best to treat the water, then add the buffer solids after autoclaving. When MES solutions are autoclaved, they turn yellow (although pH does not change measurably. The identity of the yellow breakdown product is unknown.
Easily compare specifications for MES hydrate products with the MES hydrate specification table.
Biological buffer; useful pH-range 5.8-6.5; Mobile phase component in brain tubulin isolation by amino-activated gel chromatography; Buffer in plant tissue culture.
Storage Class
13 - Non Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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M De Block
Plant physiology, 93(3), 1110-1116 (1990-07-01)
Tissue culture conditions and transformation have been established for both aspen and poplar. The use of previously described culture conditions resulted in shoot tip necrosis in the shoot cultures and necrosis of stem and leaf explants. Shoot tip necrosis could
E Lacey et al.
Journal of chromatography, 525(1), 71-84 (1990-01-26)
In the present study, we report the isolation of the acidic structural protein tubulin using a number of amino-activated gels. Crude 100,000 g supernatant derived from sheep brain was applied to gels activated with either aminohexyl, aminoethyl, argininyl, diethylaminoethyl, lysinyl
D.E. Parfitt
Sci. Hortic., 36, 157-157 (1988)
Dawson, R.M.C. et al.
Data for Biochemical Research, 410- 410 (1987)
Good NE et al.
Biochemistry, 5, 467-477 (1966)
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