biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
RAS 10, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
human, mouse, rat
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
isotype
IgG2a
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... KRAS(3845)
General description
Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/0 myeloma cells (see application references). Recognizes the ~21 kDa Ras protein.
Recognizes the ~21 kDa H-, K-, and N-Ras proteins in SW480 and Y1 cells and breast carcinoma tissue.
This Anti-Pan-Ras (Ab-3) Mouse mAb (RAS 10) is validated for use in FC, Frozen Sections, Immunoblotting, IF, IP, Paraffin Sections for the detection of Pan-Ras (Ab-3).
Immunogen
recombinant human p21 Ras
Application
Flow Cytometry (2.5 g/ml)
Frozen Sections (2.5 g/ml)
Immunoblotting (0.1-3 g/ml, chemiluminescent and colorimetric, see application references)
Immunofluorescence (2.5 g/ml, see application references)
Immunoprecipitation (1 g/sample)
Paraffin Sections (2.5 g/ml, saponin pre-treatment required)
Packaging
Please refer to vial label for lot-specific concentration.
Warning
Toxicity: Standard Handling (A)
Analysis Note
Positive Control
SW480 or Y1 cells or breast carcinoma tissue
SW480 or Y1 cells or breast carcinoma tissue
Other Notes
Rodenhuis, S. et al., 1992. Cancer Res. (Suppl)52, 2665s.
Sidransky, D., et al. 1992. Science256, 102.
Hamer, P.J., et al. 1990. Hybridoma9, 573.
Bos, J.L. 1989. Cancer Res.49, 4682.
Furth, M. E., et al. 1987. Oncogene1, 47.
Kraus, M.H., et al. 1984. Proc. Natl. Acad. Sci. USA81, 5384.
Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA80, 2112.
Taparowsky, E., et al. 1983. Cell34, 581.
Ellis, R.W., et al. 1981. Nature292, 506.
Shih, T.Y., et al. 1980. Nature287, 686.
Sidransky, D., et al. 1992. Science256, 102.
Hamer, P.J., et al. 1990. Hybridoma9, 573.
Bos, J.L. 1989. Cancer Res.49, 4682.
Furth, M. E., et al. 1987. Oncogene1, 47.
Kraus, M.H., et al. 1984. Proc. Natl. Acad. Sci. USA81, 5384.
Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA80, 2112.
Taparowsky, E., et al. 1983. Cell34, 581.
Ellis, R.W., et al. 1981. Nature292, 506.
Shih, T.Y., et al. 1980. Nature287, 686.
The level of expression of p21ras is variable in different tissues. For this reason we recommend a concentration step prior to immunoblot analysis to obtain optimal results. Antibody should be titrated for optimal results in individual systems.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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