Excitatory amino acid transporter 1 (UniProt P43003; also known as GLAST-1, Sodium-dependent glutamate/aspartate transporter 1, Solute carrier family 1 member 3) is encoded by the SLC1A3 (also known as EA6, EAAT1, GLAST, GLAST1) gene in human (Entrez Gene ID 6507). GLAST-1 is a multitransmembrane protein that functions as a high affinity glutamate transporter and mediates the termination of postsynaptic action of glutamate in central nervous system by rapidly removing released glutamate from the synaptic cleft. GLAST-1 also transports L- and D-aspartate. GLAST gene mutations are linked to episodic ataxia type 6 (EA6), a disorder characterized by seizures, migraine, and alternating hemiplegia. There are 5 known spliced variants. Isoform 2 has no functional glutamate uptake activity and exerts a dominant negative effect against isoform 1.

Clone 8C11.1 epitope lies within the second extracellular segement of GLAST isoforms 1 & 2. The epitope region is also present in isoform 4, but not in isforms 3 & 5.

Epitope: Extracellular domain

GST-tagged recombinant protein corresponding to the extracellular domain of human GLAST.

Anti-GLAST Antibody, clone 8C11.1 is an antibody against GLAST for use in Western Blotting, Immunohistochemistry .

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected GLAST in human cerebellum and rat cerebellum tissue.

Research Category
Neuroscience

Research Sub Category
Developmental Signaling

Evaluated by Western Blotting in human brain tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected GLAST in 200 µg of human brain tissue lysate.

~55 kDa observed

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1λ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Concentration: Please refer to lot specific datasheet.

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