Recognizes the full length 32 kDa caspase-3 protein. Highly specific to caspase-3 and shows no cross-reaction with other caspase family members. Caspase-3 is ubiquitously expressed and like other caspases is synthesized as an inactive proenzyme. Upon activation, caspase-3 is cleaved generating two smaller subunits of 17 kDa and 12 kDa. Caspase-3 is one of the most extensively studied enzyme in apoptosis. Activation of caspase-3 occurs in response to a variety of apoptotic inducers.

Epitope: large subunit & proform

Recombinant full-length human caspase-3 protein

Anti-Caspase 3 Antibody, large subunit & proform, clone 4-1-18 detects level of Caspase 3 & has been published & validated for use in WB, IH(P).

Research Category
Apoptosis & Cancer

Metabolism

Research Sub Category
Caspases

Enzymes & Biochemistry

Western blot: 1:1000. The antibody will detect a band at 32 kDa corresponding to the inactive unprocessed form of caspase 3 as well as a 17-22 kDa band corresponding to the large subunit of active caspase 3.

Immunohistochemistry (frozen and paraffin): 1:500.



Optimal working dilutions must be determined by end user.

Replaces: 04-439

Format: Purified

Purified immunoglobulin by protein A. Liquid in PBS, pH 7.6, with 0.1% sodium azide.

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.

Control
POSITIVE CONTROL: 293 (human embryonic kidney) cell lysates

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.