This Anti-IGF-IR (Ab-1) Mouse mAb (αIR3) is validated for use in IF, IP, Neutralization Studies, Paraffin Sections, Immunoblotting for the detection of IGF-IR (Ab-1).

Immunofluorescence (1-5 µg/ml)

Immunoprecipitation (1 µg/sample)

Neutralization Studies (1 µg/ml; see application references)

Paraffin Sections (not recommended)

Immunoblotting (not recommended)

Toxicity: Standard Handling (A)

Lyophilized from 20 mM ammonium bicarbonate solution, 100 µg BSA .

Reconstitute with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to a final concentration of 100 µg/ml. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least two h. Following reconstitution, aliquot and freeze (-20°C) or refrigerate (4°C) with 0.1% sodium azide. Freezing of aliquots is recommended for long term storage of reconstituted product. Avoid freeze/thaw cycles of solutions.

Blocks IGF-I binding to the IGF-I receptor. May also weakly cross-react with the insulin receptor (see application references). It also inhibits the growth of MCF-7 cells in culture suggesting the IGF-I receptor may be involved in autocrine regulation of cell growth (see application references). Antibody should be titrated for optimal results in individual systems.

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