Platelet Derived Growth Factor (PDGF) is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle and connective tissue. PDGF which is composed of a dimer of two chains termed the A chain and B chain, can be present as AA or BB homodimers or as an AB heterodimer. Recombinant human PDGF-BB is a 24.3 kDa disulfide-linked homodimer of two B chains (218 total amino acids).

Product Source: E. coli

For most in vitro applications, PDGF-BB exerts its biological activity in the concentration range of 0.2 to 10.0 ng/mL. Responding cells are (partial list): fibroblasts, connective tissue.

The Platelet Derived Growth Factor (PDGF) is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle & connective tissue.

Replaces: GF018

Sterile filtered solution lyophilized from 10 mM HOAc.

The sterile filtered solution was lyophilized from 10 mM HOAc.

The lyophilized PDGF-BB is stable for a few weeks at room temperature, but best stored at -20°C. After a quick spin, reconstitute in water to a concentration of 0.1-1.0 mg/mL. This solution can be diluted into aqueous buffered solutions and stored at 2-8°C for one week or -20°C for future use. Reconstituted PDGF-BB should be stored in undiluted aliquots at -20°C for up to six months.

Specific Activity: Human PDGF-BB is fully biologically active when compared to standards. The ED50 as determined by its dose-dependent mitogenic activity on BALB/c 3T3 cells is between 6-12 ng/mL.

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