Octamer-4 (Oct-4), a member of the POU family of transcription factors, has been demonstrated to be vital for the formation of self-renewing pluripotent stem cells. During embryogenesis expression of Oct-4 is limited to pluripotent cells of the inner cell mass (ICM) that contribute to the formation of all fetal cell types. This relationship between Oct-4 and pluripotency has seen this transcription factor emerge as a marker of pluripotent stem cells. Undifferentiated human and murine pluripotent embryonic stem (ES) and embryonic carcinoma (EC) cells express Oct-4. Additionally, murine embryonic germ (EG) cells are also known to express Oct-4. Following stem cell differentiation, the level of Oct-4 expression decreases. Oct-4 expression by human EG cells is not known.

Reacts with human Oct-4.

Recombinant GST fusion protein, human Oct-4 (aa 1-134).

Detect Oct-4 using this Anti-Oct-4 Antibody, clone 10H11.2, Alexa Fluor 488 conjugate validated for use in FC & IC.

Flow Cytometry:
Antibody dilution for cellular staining:
• Prepare an antibody working solution by diluting 1:5 the primary antibody with PBS.
• Dispense the volume per test of working of solution according to the number of cells indicated in the table below.
• 5 µL for Guava Flow Cytometer
• 10 µL for other Flow Cytometry instruments

Working Solution	Cells / test	Total Reaction Volume
5 μl	5 x 105 Cells / 95 μl PBS	100 μl
10 μl	1 x 106 Cells / 90 μl PBS	100 μl

Research Category
Stem Cell Research

Research Sub Category
Pluripotent & Early Differentiation

Evaluated by Flow Cytometry in 2102Ep Cells.

~44 kDa

Purified mouse monoclonal IgG1 conjugated to Alexa Flour 488 in PBS with less than 0.09% sodium azide and 15 mg/mL BSA.

Maintain refrigerated at 2-8°C protected from light in undiluted aliquots for up to 6 months from date of receipt

Control
Human embryonic stem cells

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