Human neutral endopeptidase (Neprilysin) is single pass type II integral membrane endopeptidase that functions to activate and degrade biologically active enzymes. Endopeptidase,in general, function to cleave peptide bonds within enzyme sequences, in contrast to exopeptidases that target the end sequence. Neprilysin is part of the peptidase M13 family that acts on polypeptides of less than 30 amino acids with Thermolysin-like specificity for cleave sites. Thermolysin is thought to cleave before amino acids Ile, Met, Phe, Trpm or Val (unless Pro precedes) and after Ala, Asp, His, or Thr. Neprilysin preferentially cleaves polypeptides between hydrophobic residues, particularly with Phe or Tyr at position 1. Neprilysin research has found it important in the degradation of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Research has found it may function in the degradation of atrial natriuretic factor (ANF). Upregulation of ANF functions to regulate homeostatis of water, sodium, potassium and adipose in response to high blood pressure in muscle cells of the heart.

Reactivity with other species has not been confirmed.

Recognizes Neprilysin (Neutral Endopeptidase, NEP). On western blots of rat kidney extracts the antibody detects a band migrating at approximately 86 kDa corresponding to neprilysin.

Rat Neprilysin.

Research Category
Neuroscience

Research Sub Category
Neurodegenerative Diseases

This Anti-Neprilysin Antibody (Neutral Endopeptidase, Nep) is validated for use in IH, WB for the detection of Neprilysin (Neutral Endopeptidase).

Western Blotting:
A previous lot of this antibody was used on Western Blotting.

Immunohistochemistry:
A previous lot of this antibody was used on Immunohistochemistry.

Optimal working dilutions must be determined by the end user.

~86 kDa

Rabbit polyclonal serum. Contains no preservative.

Unpurified

Stable for 1 year at -20ºC from date of receipt.

Control
Kidney tissue.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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