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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
mouse
Quality Level
conjugate
ALEXA FLUOR™ 647
antibody form
purified antibody
antibody product type
primary antibodies
clone
3H10, monoclonal
species reactivity
human
technique(s)
immunocytochemistry: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
phosphorylation (pSer10)
Gene Information
human ... HIST1H3F(8968)
General description
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. Histone proteins are highly post-translationally modified, and Histone H3 is the most extensively modified of the five histones. Histone H3 sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes. Di- and tri-methylation of histone H3 are gene silencing marks, while removal of these marks by the demethylase genes Jmjd1a and Jmjd2c leads to gene activation. This product is a conjugated version of Cat. No. 05-806.
Immunogen
Linear peptide corresponding to Histone H3 phosphorylated at Ser10.
Application
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
This Anti-phospho Histone H3 (Ser10) antibody, clone 3H10, Alexa Fluor™ 647 is a purified mouse monoclonal for detection of Histone H3 phosphorylated at serine 10.
This mAb is fluorescently labeled and validated for use in IC. The unconjugated version has been proven in FC, ICC, IF, PIA, WB, Mplex.
This mAb is fluorescently labeled and validated for use in IC. The unconjugated version has been proven in FC, ICC, IF, PIA, WB, Mplex.
Quality
Evaluated by Immunocytochemistry Analysis in HeLa cells.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected phospho Histone H3 (Ser10) in HeLa cells.
Alexa Fluor™ is a registered trademark of Life Technologies.
Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected phospho Histone H3 (Ser10) in HeLa cells.
Alexa Fluor™ is a registered trademark of Life Technologies.
Target description
~17 kDa observed (Refer to Cat. No. 05-806)
Physical form
Protein G Purified
Purified mouse conjugated antibody in buffer containing PBS and 15 mg/ml BSA with 0.1% Sodium Azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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