Gemin3 is a member of the DEAD box protein family. The members of this family are characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD). Some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. Gemin 3 is a component of the survival of motor neurons (SMN) complex and codes for a DEAD box protein with an ATPase activity The SMN complex is essential for the biogenesis of spliceosomal small nuclear ribonucleoproteins and likely functions in the assembly, metabolism, and transport of a diverse number of other ribonucleoproteins. Specifically, the SMN complex assembles 7 Sm proteins (Gemin2-8) into a core structure around a highly conserved sequence of ribonucleic acid (RNA) found in small nuclear RNAs. The complex recognizes specific sequences and structural features of small nuclear RNAs and Sm proteins and assembles small nuclear ribonucleoproteins in a stepwise fashion.

This antibody recognizes Gemin-3.

Epitope: Unknown

Recombinant protein corresponding to human Gemin-3.

Anti-Gemin3 Antibody, clone 12H12 is a Mouse Monoclonal Antibody for detection of Gemin3 also known as Component of gems 3, DEAD-box protein DP103 & has been validated in WB.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
RNA Metabolism & Binding Proteins

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.5 µg/ml of this antibody detected Gemin-3 on 10 µg of HeLa cell lysate.

Detects a band of approximately 105 kDa (Calculated molecular weight: 92 kDa)

Format: Purified

Protein G Purified

Purified mouse monoclonal IgGκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
HeLa cell lysate

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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