GC Analysis of Trichloroanisole (TCA) and Precursors in Red Wine (Spiked) on SLB-5ms after SPME using 100 μm PDMS Fiber

sample/matrix

1.5 mL red wine spiked with each analyte at 100 ng/L + 600 μL 5% potassium carbonate (K₂CO₃) + 240 mg sodium chloride (NaCl) + 60 μL acetic anhydride

SPME fiber

metal fiber coated with 100 μm PDMS (57928-U)

extraction

headspace, 50 °C for 30 min, with stirring

desorption process

250 °C for 3 min

column

SLB-5ms, 30 m x 0.25 mm I.D., 0.25 μm (28471-U)

oven

50 °C (1 min), 25 °C/min to 280 °C

detector

ECD, 290 °C

carrier gas

helium, 1.5 mL/min, constant

liner

0.75 mm I.D. SPME