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  • Non-covalent photo-patterning of gelatin matrices using caged collagen mimetic peptides.

Non-covalent photo-patterning of gelatin matrices using caged collagen mimetic peptides.

Macromolecular bioscience (2014-12-06)
Yang Li, Boi Hoa San, Julian L Kessler, Jin Hwan Kim, Qingguo Xu, Justin Hanes, Seungju Michael Yu
ABSTRACT

To address the downside of conventional photo-patterning which can alter the chemical composition of protein scaffolds, we developed a non-covalent photo-patterning strategy for gelatin (denatured collagen) hydrogels that utilizes UV activated triple helical hybridization of caged collagen mimetic peptide (caged CMP). Here we present 2D and 3D photo-patterning of gelatin hydrogels enabled by the caged CMP derivatives, as well as creation of concentration gradients of CMPs. CMP's specificity for binding to gelatin allows patterning of almost any synthetic or natural gelatin-containing matrix, such as gelatin-methacrylate hydrogels and corneal tissues. This is a radically new tool for immobilizing drugs to natural tissues and for functionalizing scaffolds for complex tissue formation.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
1,2-Dichloroethane, anhydrous, 99.8%
Sigma-Aldrich
Piperidine solution, suitable for peptide synthesis, 20% in DMF
Supelco
Dimethyl sulfoxide, analytical standard
Supelco
Trifluoroacetic acid, analytical standard
Sigma-Aldrich
N,N-Diisopropylethylamine, ReagentPlus®, ≥99%
Sigma-Aldrich
Dimethyl sulfoxide, Vetec, reagent grade, 99%
Sigma-Aldrich
Dimethyl sulfoxide, ReagentPlus®, ≥99.5%
Sigma-Aldrich
Trifluoroacetic acid, ≥99%, for protein sequencing
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1,2-Dichloroethane, ACS reagent, ≥99.0%
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Dimethyl sulfoxide, puriss. p.a., dried, ≤0.02% water
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Dimethyl sulfoxide, ACS reagent, ≥99.9%
Sigma-Aldrich
Dimethyl sulfoxide, suitable for HPLC, ≥99.7%
Sigma-Aldrich
Trifluoroacetic acid, ReagentPlus®, 99%
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Dimethyl sulfoxide, puriss. p.a., ACS reagent, ≥99.9% (GC)
Supelco
Dimethyl sulfoxide, for inorganic trace analysis, ≥99.99995% (metals basis)
Dimethyl sulfoxide, European Pharmacopoeia (EP) Reference Standard
USP
Dimethyl sulfoxide, United States Pharmacopeia (USP) Reference Standard
Supelco
Fmoc chloride, for HPLC derivatization, LiChropur, ≥99.0% (HPLC)
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Fmoc-Cys(Trt)-OH, ≥95.0% (sum of enantiomers, HPLC)
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N-Hydroxysuccinimide, purum, ≥97.0% (T)
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N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide, ≥97.0% (T)
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HBTU, ≥98.0% (T)
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Ethanesulfonic acid, 95%
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N-Hydroxysuccinimide, 98%
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5(6)-Carboxyfluorescein, Dye content 90 %
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Dimethyl sulfoxide, BioUltra, for molecular biology, ≥99.5% (GC)
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2-Pyrrolidinone, purum, ≥98.0% (GC)
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2-Pyrrolidinone, ≥99%
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Trifluoroacetic acid, ReagentPlus®, 99%
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Triisopropylsilane, 98%