- Photoaffinity casting of a coumarin flag for rapid identification of ligand-binding sites within protein.
Photoaffinity casting of a coumarin flag for rapid identification of ligand-binding sites within protein.
Chemical communications (Cambridge, England) (2013-01-26)
Shota Morimoto, Takenori Tomohiro, Nobuyuki Maruyama, Yasumaru Hatanaka
PMID23349004
ABSTRACT
A photo-switchable fluorescent flagging approach has been developed to identify photoaffinity-labeled peptides in target protein. Upon photochemical release of the ligand, the protein was newly modified with a coumarin in place of the previously attached biotin. It allowed us to simplify complex identification processes for labeled sites.
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Coumarin, primary reference standard
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Supelco
Coumarin, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland
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