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  • The combined effect of mesenchymal stem cells and resveratrol on type 1 diabetic neuropathy.

The combined effect of mesenchymal stem cells and resveratrol on type 1 diabetic neuropathy.

Experimental and therapeutic medicine (2019-04-17)
Chen Wang, Jingwei Chi, Kui Che, Xiaolong Ma, Mingyue Qiu, Zhongchao Wang, Yangang Wang
ABSTRACT

Diabetic neuropathy (DN) is one of the most common diabetic complications that results in an increase in patient discomfort and pain. The present study demonstrated that mesenchymal stem cells (MSCs) or resveratrol (RSV) may improve diabetic hyperglycemia and neuropathy. The aim of the present study was to investigate the combined effect of MSCs and RSV on DN. A total of 100 non-obese diabetic mice were divided into the following six groups: Normal control, MSCs, RSV, MSCs + RSV, insulin and diabetic control groups. Following homologous therapy, the levels of blood glucose and C-peptide, islets, nuclear factor (NF)-κB, nerve growth factor (NGF) and myelin basic protein (MBP), and the sciatic nerve structure in each group were examined and evaluated. Following the administration of therapy, the levels of blood glucose and C-peptide in mice in the MSCs + RSV group were significantly improved when compared with the other diabetic groups, and the dosage of insulin therapy required was the lowest among the six experimental groups (P<0.05). The levels of NGF, MBP and NF-κB in the MSCs + RSV group were significantly improved compared with the MSCs and RSV groups (P<0.05). Furthermore, the diameter of the axon, number of myelinated nerve fibers and the depth of the myelin sheath in the MSCs + RSV group were greatest among the five examined groups (excluding the control). The combination of RSV and MSCs could relieve hyperglycemia and improve DN. This indicated that the combination of RSV and MSCs may be a novel therapeutic method for the treatment of DN.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Anti-Mouse IgG1 (heavy chain specific) antibody produced in goat, affinity isolated antibody, lyophilized powder
Sigma-Aldrich
Indomethacin, 98.5-100.5% (in accordance with EP)
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3-Isobutyl-1-methylxanthine, ≥99% (HPLC), powder
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Monoclonal Anti-Insulin antibody produced in mouse, clone K36AC10, ascites fluid
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Monoclonal Anti-MBP antibody produced in mouse, Prestige Antibodies® Powered by Atlas Antibodies, clone CL2819, purified immunoglobulin, buffered aqueous glycerol solution
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Resveratrol, ≥99% (HPLC)
Sigma-Aldrich
Monoclonal Anti-Nerve Growth Factor-β antibody produced in mouse, clone 25623, purified immunoglobulin, lyophilized powder