SRP3211
IL-4 from mouse
Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture
Synonym(s):
B cell stimulating factor (BSF-1), BCDF, BCGF
Sign Into View Organizational & Contract Pricing
Select a Size
All Photos(1)
Select a Size
Change View
About This Item
UNSPSC Code:
12352202
NACRES:
NA.32
Recommended Products
biological source
mouse
recombinant
expressed in E. coli
Assay
≥98% (HPLC)
≥98% (SDS-PAGE)
form
lyophilized
potency
≤2.0 ng/mL ED50
mol wt
13.7 kDa
packaging
pkg of 20 μg
technique(s)
cell culture | mammalian: suitable
impurities
<0.1 EU/μg endotoxin, tested
color
white to off-white
General description
IL-4 (interleukin 4) is a pleiotropic cytokine. Produced by mast cells, T cells and bone marrow stromal cells. Recombinant murine IL-4 is a 13.7kDa globular protein containing 120 amino acid residues.
Biochem/physiol Actions
It regulates diverse T and B cell responses including cell proliferation, survival and gene expression. IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 (T helper) cells, characterized by their cytokine-secretion profile that includes secretion of IL-4, IL-5, IL-6, IL-10, and IL-13, which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 (immunoglobulin G1) and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergy. IL-4 helps in long-term recovery after cerebral ischemia by controlling the activities of microglia/macrophage. In mice, absence of IL-4 is associated with a state anxiety-like behavior.
Sequence
MHIHGCDKNH LREIIGILNE VTGEGTPCTE MDVPNVLTAT KNTTESELVC RASKVLRIFY LKHGKTPCLK KNSSVLMELQ RLFRAFRCLD SSISCTMNES KSTSLKDFLE SLKSIMQMDY S
Physical form
Lyophilized with no additives.
Reconstitution
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20°C to -80°C.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Choose from one of the most recent versions:
Certificates of Analysis (COA)
Lot/Batch Number
Don't see the Right Version?
If you require a particular version, you can look up a specific certificate by the Lot or Batch number.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Chih-Wei Ko et al.
Bio-protocol, 8(17), e3003-e3003 (2018-09-05)
Using gas chromatography mass spectrometry (GC-MS) to analyze the citric acid cycle (CAC) and related intermediates (such as glutamate, glutamine, GABA, and aspartate) is an analytical approach to identify unexpected correlations between apparently related and unrelated pathways of energy metabolism.
Mast cell production and response to IL-4 and IL-13.
McLeod JJ
Cytokine, 75, 57-61 (2015)
Cell division number regulates IgG1 and IgE switching of B cells following stimulation by CD40 ligand and IL-4.
Hasbold J
European Journal of Immunology, 28, 1040-1051 (1998)
Nasal mast cells in perennial allergic rhinitics exhibit increased expression of the Fc epsilonRI, CD40L, IL-4, and IL-13, and can induce IgE synthesis in B cells.
Pawankar R
The Journal of Clinical Investigation, 99, 1492-1492 (1997)
IL-4 Knock Out Mice Display Anxiety-Like Behavior.
Moon ML
Behavior Genetics, 45, 451-460 (2015)
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service