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SRP0733

Sigma-Aldrich

PCTK2 (CDK17)/CYCLINY, Active

PRECISIO® Kinase, recombinant

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.77

recombinant

expressed in baculovirus infected Sf9 cells

Assay

70-95% (SDS-PAGE)

form

frozen liquid

specific activity

15-25 nmol/min·mg (radiometric)

mol wt

~64 kDa (Cyclin Y)
 ~88 kDa (PCTK2)

concentration

0.1 μg/μL

accession no.

NM_002595
NM_145012

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... CCNY(219771) , CDK17(5128)

Application

Useful for kinase assay and Western blot.

Biochem/physiol Actions

Recombinant full-length human PCTK2 (CDK17) and Cyclin Y (2-end) were co-expressed by Baculovirus in Sf9 insect cells using N-terminal GST tags. PCTK2 (CDK17) or cyclin-dependent kinase 17 belongs to the cdc2/cdkx subfamily of the ser/thr family of protein kinases. PCTK2 has similarity to a rat protein that is thought to play a role in terminally differentiated neurons (1). The identification of a large family of cdc2-related kinases opens the possibility of combinatorial regulation of the cell cycle together with the emerging large family of cyclins (2).

Reconstitution

Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.

Storage and Stability

Store in aliquots at -70°C. Avoid freeze/thaw cycles.

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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