Plant Culture Media
Growing plant tissue in the laboratory may include seed, meristem, callus, and bud culture, and requires specialized plant culture media. Murashige and Skoog (also called MS media, MSO, or MS0) and Gamborg’s B5 medium are two of the most essential media formulations used for culturing plants. These media contain all the micro- and macronutrients, vitamins, organic supplements, and plant growth regulators that are necessary for the growth and multiplication of plant cells, tissues, and organs in vitro. For applications that require more custom formulations, we offer a comprehensive selection of basal salt mixtures, vitamin solutions, and supplements. test2304
Products
Murashige and Skoog (MS Media)
Much of plant tissue culture for research and biotechnology still relies on media described more than 50 years ago by Murashige and Skoog. Their eponymous Murashige and Skoog media, or MS media, is the most widely-used plant culture medium and is available as basal salt mixtures or media containing organics.
Regardless of format, MS media is comprised of micro- and macronutrients, plus nutrients like sugar, vitamins, and growth regulators. Different MS media components can be custom-combined to create the right media formulation for each plant cell or tissue type.
Gamborg’s B5 Medium
Gamborg′s B5 medium is an optimized culture medium for in vitro plant cell, tissue, and organ culture. It contains a mixture of inorganic salts, vitamins, and carbohydrates. We offer Gamborg’s B5 medium with minimal organics, Gamborg’s vitamin mix, and Gamborg’s B5 basal salt mix.
Phytamax™ Orchid Culture Medium
Phytamax™ Orchid Multiplication Medium and Phytamax™ Orchid Maintenance Medium are used for culturing, propagating and replating the orchid stem. Phytamax™ orchid culture media are available with or without activated charcoal, and with both charcoal and banana powder.
Related Resources
- Article: Antibiotics in Plant Tissue Culture Protocol
Przewodnik referencyjny i przewodnik przygotowania do stosowania antybiotyków i środków przeciwgrzybiczych w hodowli tkanek roślinnych.
- Article: Classical Plant Media Formulations
Klasyczne pożywki do hodowli tkanek roślinnych opracowane wiele lat temu przez pionierów takich jak Murashige, Skoog, Gamborg i innych nadal odgrywają istotną rolę w badaniach nad kulturami tkanek roślinnych.
- Article: Explant Sterilization – Plant Tissue Culture Protocol
Sterylizacja powierzchniowa materiału roślinnego jest ważna dla hodowli tkanek roślinnych. Uwzględniono powszechnie stosowane środki dezynfekujące do hodowli tkanek roślinnych oraz metody dezynfekcji nasion.
- Article: Growth Regulators – Plant Tissue Culture Protocol
Skorzystaj z tabel w tym protokole, aby pomóc w przygotowaniu auksyn, cytokin i innych regulatorów do stosowania w pożywkach do hodowli komórek roślinnych.
- Article: Media Preparation – Plant Tissue Culture Protocol
Preparation from Packaged Powder, Preparation from Basal Salt Solutions, Banana Powder Preparation and Use, Coconut Water Preparation and Use
- Article: Pathology Media – Plant Tissue Culture Protocol
Fungal and Bacterial Detection Media, Media Preparation and Storage, Media Composition Table.
- Article: Phycology Media in Plant Tissue Culture
Phycology media can be prepared from concentrated solutions or from powdered salt mixtures. The concentrated solutions are complete, including vitamins, and should be stored frozen.
- Brochure: Plant Tissue Culture Products to Ensure Your Success
Sigma-Aldrich’s combination of qualified plant tissue culture products and global business capabilities help customers maintain reagent consistency and suitability.
- Article: Silver Thiosulfate – Plant Tissue Culture Protocol
Instrukcje przygotowania 0,1 M roztworów podstawowych tiosiarczanu sodu (STS) i 0,1 M azotanu srebra do użytku laboratoryjnego.
- Article: Vitamin Mixtures – Plant Tissue Culture Protocol
Preparation of Solutions from Packaged Powder, Media Preparation using Vitamin Solutions, Vitamin Mixtures Composition Table
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