P5380

Protease from Bacillus licheniformis

Name: Protease from <I>Bacillus licheniformis</I>
Brand: SIGMA

Type VIII, lyophilized powder, 7-15 units/mg solid

Synonim(y):

Proteinaza z Bacillus licheniformis, Subtylizyna A

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About This Item

CAS Number:

9014-01-1

Numer WE:

3.4.21.62 (BRENDA, IUBMB)

Numer WE:

232-752-2

Numer MDL:

MFCD00132092

Kod UNSPSC:

12352204

NACRES:

NA.54

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Pozwól nam pomóc

pochodzenie biologiczne

Bacillus licheniformis

Poziom jakości

200

typ

Type VIII

Formularz

lyophilized powder

aktywność właściwa

7-15 units/mg solid

masa cząsteczkowa

27 kDa

oczyszczone przez

crystallization

rozpuszczalność

10 mM NaAc (pH 7.5) and 5 mM CaAc: soluble, clear
H₂O: soluble, colorless

obecność zanieczyszczeń

DNase ≤5.0 Kunitz units/mg solid
RNase ≤0.05 Kunitz units/mg solid

temp. przechowywania

−20°C

Opis ogólny

The enzyme is found to be stable at pH 8-10, retaining activity of up to 90% for 24 hours. It shows maximum activity at temperatures between 55-60 °C.

Specyficzność

Subtilisin A is a member of the Serine S8 Endoproteinase family. It has broad specificity with a preference for a large uncharged residue in the P1 position. It hydrolyzes native and denatured proteins, and is active under alkaline conditions.

Zastosowanie

Non-specific Protease Activity Assay Video

The product has been used with other enzymes for in situ proteolysis to produce crystals suitable for structure determination. It has also been used in the process of isolation of subsarcolemmal (SS) and intermyofibrillar (IMF) mitochondria that can be used for functional in vitro studies.

This is a proteolytic enzyme isolated from the fermentation of Bacillus licheniformis. It is a serine endoproteinase with a broad specificity towards native and denatured proteins, and is active under alkaline conditions.

Działania biochem./fizjol.

Protease catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate. Protease, Product P5380, is a serine endoproteinase with a broad specificity towards native and denatured proteins, and is active under alkaline conditions. It is active in some organic solvents such as dry octane.

Właściwości fizyczne

Subtilisin is a non-glycosylated single polypeptide chain without disulfide bonds and has a molecular weight of 27 KDa.

Definicja jednostki

One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).

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