P0114

Phosphatase, Alkaline from bovine intestinal mucosa

BioUltra, ≥5,700 DEA units/mg protein

• Synonim(y): Alkaline phosphatase, Orthophosphoric-monoester phosphohydrolase (alkaline optimum)
• CAS Number: 9001-78-9
• Numer WE: 3.1.3.1 (BRENDA, IUBMB)
• Numer MDL: MFCD00131849
• Kod UNSPSC: 12352204
• eCl@ss: 42010105
• NACRES: NA.54

Właściwości

• linia produktu: BioUltra
• Poziom jakości: 200
• Formularz: (40% glycerol containing 6 mM Tris, 6 mM MgCl2 and 0.12 mM ZnCl2, pH approximately 7.6)
• aktywność właściwa: ≥5,700 DEA units/mg protein
• masa cząsteczkowa: dimer ~160 kDa
• stężenie: ≥10 mg/mL
• Warunki transportu: wet ice
• temp. przechowywania: 2-8°C

Opis

Opis ogólny

Alkaline phosphatase from bovine intestinal mucosa is both a phosphomonoesterase and a pyrophosphatase.

Zastosowanie

Alkaline phosphatase can be used to dephosphorylate casein and other proteins. Alkaline phosphatase may be also be used to dephosphorylate the 5′-termini of DNA or RNA to prevent self-ligation. DNA or RNA can also be tagged with radiolabeled phosphate (via T4 polynucleotide kinase) after dephosphorylation with alkaline phosphatase..

High specific activity grade recommended for antibody and protein conjugation.

Phosphatase alkaline from bovine intestinal mucosa has been used in a study to measure the hydrolysis kinetics of three polyfluorinated alkyl phosphate monoesters, differing in fluorinated chain length. It has also been used in a study to show that the developmentally controlled expression of the isosomes C-1 and C-2 indicate functional changes in intestinal alkaline phosphatase with tissue maturation.

Działania biochem./fizjol.

Alkaline phosphatase was shown to have anti-inflammatory effects and can reduce the severity of septic shock by detoxifying lipid A.

The enzyme has a broad specificity for phosphate esters of alcohols, amines, pyrophosphate, and phenols. It is routinely used to dephosphorylate proteins and nucleic acids.

Właściwości fizyczne

Bovine intestinal alkaline phosphatase is a dimeric, membrane-derived glycoprotein. At least three isoforms exist, which typically possess two N-linked and one or more O-linked glycans per monomer.

Bovine intestinal alkaline phosphatase is a dimeric, membrane-derived glycoprotein. At least three isoforms exist, which typically possess two N-linked and one or more O-linked glycans per monomer.2 The enzyme requires zinc, and magnesium or calcium divalent ions for activity.

Definicja jednostki

One DEA unit will hydrolyze 1 μmole of 4-nitrophenyl phosphate per minute at pH 9.8 at 37 °C. (One glycine unit is equivalent to ~3 DEA units)

Postać fizyczna

Solution in 40% glycerol containing 6 mM Tris, 6 mM MgCl₂ and 0.12 mM ZnCl₂, pH approximately 7.6

Komentarz do analizy

Package sizes are based on DEA units

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS08
• Hasło ostrzegawcze: Danger
• Zwroty wskazujące rodzaj zagrożenia: H334
• Zwroty wskazujące środki ostrożności: P261 - P284 - P501
• Klasyfikacja zagrożeń: Resp. Sens. 1
• Kod klasy składowania: 10 - Combustible liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)