CAPHOS

Calcium Phosphate Transfection Kit

Most cost effective transfection reagent kit for transient and stable transfection of DNA into mammalian cells

• Synonim(y): Dostarczanie genów
• Numer WE: 233-140-8
• Kod UNSPSC: 12352200
• NACRES: NA.85

Właściwości

• klasa czystości: Molecular Biology
• Poziom jakości: 200
• Formularz: solution
• zastosowanie: kit sufficient for 160 transfections (6 cm dishes); kit sufficient for 400 transfections (3.5 cm dishes); kit sufficient for 80 transfections (10 cm dishes)
• metody: transfection: suitable
• Warunki transportu: dry ice
• temp. przechowywania: −20°C

Opis

Opis ogólny

Calcium phosphate transfection is a commonly used method for the introduction of DNA into eukaryotic cells. This technique has been used to obtain both transient and stable transfections in a wide variety of cell types.

Zastosowanie

Calcium Phosphate Transfection Kit has been used:

• to enable transfection
• to transfect Hek293T cells
• to transfect H29D cells

Suitable for transient and stable transfection of DNA into cultured mammalian cells. The following cells have successfully been transfected using the calcium phosphate method:

BAEC
Bowels melanoma cells
CHO K1
COS-7
Fibroblasts (human embryonic, neo derm)
HEK293
Huh 7
IMR-90
LLC (Lewis Lung Carcinoma)
NIH3T3
PC-12
PCI-13
SH-Sy5Y
SK-Hep-1
T47D

Cechy i korzyści

• Suitable for transient and stable transfection
• Reproducible for a wide range of cell types
• Widely referenced
• Inexpensive

Komponenty

The Calcium Phosphate Transfection Kit contains:
5 ml 2.5M CaCl2 (C2052)
25 ml 2x HEPES Buffered Saline (H1012)
25 ml molecular biology grade water (W4502)

Zasada

The procedure is based on slow mixing of HEPES-buffered saline containing sodium phosphate with a CaCl₂ solution containing the DNA. A DNA-calcium phosphate co-precipitate forms, which adheres to the cell surface and is taken up by the cell, presumably by endocytosis.

Inne uwagi

The protocol was developed for transient transfection of CHO cells using pSV40-CAT plasmid as a reporter gene.

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS07
• Hasło ostrzegawcze: Warning
• Zwroty wskazujące rodzaj zagrożenia: H319
• Zwroty wskazujące środki ostrożności: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Klasyfikacja zagrożeń: Eye Irrit. 2
• Kod klasy składowania: 12 - Non Combustible Liquids
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable