C6079

Collagenase + protease inhibitor

2-5 FALGPA units/mg solid, ≥800 CDU/mg solid

• Synonim(y): Preparat kolagenazy, Roztwór inhibitora proteazy
• Numer WE: 3.4.24.3 (BRENDA, IUBMB)
• Kod UNSPSC: 12352204
• NACRES: NA.54

Właściwości

• pochodzenie biologiczne: bacterial (Clostridium histolyticum)
• Poziom jakości: 200
• Formularz: solid
• aktywność właściwa: ≥800 CDU/mg solid; 2-5 FALGPA units/mg solid
• masa cząsteczkowa: 68-130 kDa
• rozpuszczalność: water: soluble
• Warunki transportu: dry ice
• temp. przechowywania: −20°C
• informacje o genach: rat ... LOC296948(296948)

Opis

Zastosowanie

Collagenase with protease inhibitor has been used in a study to assess the association of immune system gene polymorphisms with quantitative features. It has also been used in a study to investigate the quantitative structure-activity relationship study on Clostridium histolyticum collagenase inhibitors. The enzyme from sigma has been used in the isolation of porcine pancreatic islets. It has also been used in the isolation of pancreatic-infiltrating lymphocytes from mice.

Działania biochem./fizjol.

Effective release of cells from tissue requires the action of both collagenase enzymes and the neutral protease. Collagenase is activated by four grams of calcium (Ca²⁺) per mole of the enzyme. The culture filtrate is thought to contain at least 7 different proteases ranging in molecular weight from 68-130 kDa. The pH optimum is 6.3-8.8. The enzyme is typically used to digest the connective components in tissue samples to liberate individual cells. Collagenase treatment can cause some cells to die. Typically, concentrations varying from 0.1 to 5 mg/mL are used for digestion. The duration of reaction can vary from 15 minutes to several hours for satisfactory cell dissociation without causing too much cell death. Zn²⁺ is required for activity. This product is used if the collagenase does not require a significant protease activity.

Inne uwagi

Selected lots of collagenase Type XI blended with protease inhibitor to limit the tryptic enzyme activities in pancreatic tissue. The formulation was developed through collaboration with several outside laboratories.

Jakość

Also contains clostripain and non-specific neutral protease activities.

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS08,GHS07
• Hasło ostrzegawcze: Danger
• Zwroty wskazujące rodzaj zagrożenia: H302 + H312 + H332,H317,H334
• Zwroty wskazujące środki ostrożności: P261 - P264 - P271 - P280 - P301 + P312 - P302 + P352 + P312
• Klasyfikacja zagrożeń: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Resp. Sens. 1 - Skin Sens. 1
• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: dust mask type N95 (US), Eyeshields, Faceshields, Gloves