HPLC Analysis of Fusion Protein on Zenix®-C SEC-300, Effect of Mobile Phase Composition
Materials
analytical column
Product No.
Description
Pricing
Zenix®-C SEC-300 Gel Filtration Column, 3 μm
L × I.D. 30 cm × 7.8 mm, 3 μm particle size, stainless steelCONDITIONS
column
Zenix-C SEC-300, 30 cm x 7.8 mm I.D., 3 μm particles (Z777092)
gradient
(A) lower, 150 mM sodium phosphate buffer, pH 7.0 (B) middle, 150 mM sodium phosphate buffer, pH 7.0 + 10% acetonitrile (C) top, 150 mM sodium phosphate buffer, pH 7.0 + 20 mM NaCl
flow rate
0.5 mL/min
column temp.
25 °C
detector
UV, 214 nm
injection
10 μL
sample
1 mg/mL fusion protein, MW 170 kD, pl 6.8-7.0
Description
Legal Information
Zenix is a registered trademark of Sepax Technologies