Characterization of ceramic hydroxyapatite surface by inverse liquid chromatography in aquatic systems.

Characterization of ceramic hydroxyapatite surface by inverse liquid chromatography in aquatic systems.

Talanta (2015-11-26)

Karol Kadlec, Katarzyna Adamska, Adam Voelkel

ABSTRACT

The novel approach for hydroxyapatite (HA) surface characterization was proposed. The main aim of this investigation was to estimate surface properties of HA as a biomaterial in real system i.e. in simulated body fluid (SBF). One of the method, which might be used to reflect the influence of liquid environment on sorption properties of material being surrounded by this liquid, is called inverse liquid chromatography (ILC). The lowercase letters of LFER equation (e, s, a, b, v) served for this characterization. The sorption abilities of examined material were also estimated for two different aqueous mobile phases: deionized water and water solution of 0.1M Na2HPO4. It enabled to observe the change in physiochemical properties of surface, considered in Abraham model, dependence on ions concentration in the mobile phase. Moreover pH of every aquatic solution, normally about 7, was adjusted to 5.5 and 9 to observe the influence of hydrogen and hydroxyl ions concentration on HA sorption properties.

MATERIALS

Product Number

Brand

Product Description

04238

Sigma-Aldrich

Hydroxyapatite, puriss., meets analytical specification of Ph. Eur., BP, FCC, E341, ≥90% (calculated on glowed substance)

P4504

Sigma-Aldrich

Potassium chloride, ReagentPlus^®, ≥99.0%

60142

Sigma-Aldrich

Potassium chloride solution, BioUltra, ~1 M in H₂O

204099

Sigma-Aldrich

Potassium chloride, ≥99.99% trace metals basis

451029

Sigma-Aldrich

Potassium chloride, AnhydroBeads^™, −10 mesh, 99.99% trace metals basis

P9666

Sigma-Aldrich

Potassium phosphate dibasic trihydrate, ≥99%

P9333

Sigma-Aldrich

Potassium chloride, BioXtra, ≥99.0%

P5504

Sigma-Aldrich

Potassium phosphate dibasic trihydrate, ReagentPlus^®, ≥99.0%

793590

Sigma-Aldrich

Potassium chloride, anhydrous, free-flowing, Redi-Dri^™, ReagentPlus^®, ≥99%

P9541

Sigma-Aldrich

Potassium chloride, ≥99.0%

746436

Sigma-Aldrich

Potassium chloride, anhydrous, free-flowing, Redi-Dri^™, ACS reagent, ≥99%

60130

Sigma-Aldrich

Potassium chloride, puriss. p.a., ≥99.5% (AT)

12636

Sigma-Aldrich

Potassium chloride, puriss., meets analytical specification of Ph. Eur., BP, USP, FCC, E508, 99-100.5% (AT), ≤0.0001% Al

449989

Sigma-Aldrich

Potassium chloride, AnhydroBeads^™, −10 mesh, 99.999% trace metals basis

409316

Sigma-Aldrich

Potassium chloride, 99.999% trace metals basis

60128

Sigma-Aldrich

Potassium chloride, BioUltra, ≥99.5% (AT)

P5405

Sigma-Aldrich

Potassium chloride, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥99.0%

P3911

Sigma-Aldrich

Potassium chloride, ACS reagent, 99.0-100.5%

289396

Sigma-Aldrich

Hydroxyapatite, reagent grade, powder, synthetic

21223

Sigma-Aldrich

Hydroxyapatite, purum p.a., ≥90% (as Ca₃(PO₄)₂, KT)

702153

Sigma-Aldrich

Hydroxyapatite, nanoparticles, dispersion, 10 wt. % in H₂O, <200 nm particle size (BET)

574791

Sigma-Aldrich

Hydroxyapatite, synthetic, 99.8% trace metals basis (excludes Mg)

677418

Sigma-Aldrich

Hydroxyapatite, nanopowder, <200 nm particle size (BET), ≥97%, synthetic

33209

Sigma-Aldrich

Acetic acid, glacial, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.8%

S5640

Sigma-Aldrich

Sodium sulfate, ≥99.0%, suitable for plant cell culture

V800400

Sigma-Aldrich

Sodium sulfate, AR, anhydrous, ≥99.5%

13464

Sigma-Aldrich

Sodium sulfate, puriss., meets analytical specification of Ph. Eur., BP, USP, anhydrous, 99.0-100.5% (calc. to the dried substance)

793531

Sigma-Aldrich

Sodium sulfate, anhydrous, free-flowing, Redi-Dri^™, ReagentPlus^®, ≥99%

378860

Sigma-Aldrich

Sodium chloride, random crystals, optical grade, 99.9% trace metals basis

P9327

Sigma-Aldrich

Potassium chloride solution, 0.075 M, sterile-filtered, BioXtra, suitable for cell culture