D7008

Dihydroethidium

≥95% purity, powder

• Synonym(s): 2,7-Diamino-10-ethyl-9-phenyl-9,10-dihydrophenanthridine, 3,8-Diamino-5,6-dihydro-5-ethyl-6-phenylphenanthridine, Hydroethidine
• Empirical Formula (Hill Notation): C₂₁H₂₁N₃
• CAS Number: 104821-25-2
• Molecular Weight: 315.41
• Beilstein: 5107482
• MDL number: MFCD00077335
• UNSPSC Code: 12171500
• PubChem Substance ID: 24894069
• NACRES: NA.47

Properties

• Product Name: Dihydroethidium, ≥95%
• Quality Level: 100
• Assay: ≥95%
• form: powder
• technique(s): titration: suitable
• color: pink to very dark red
• solubility: chloroform: 20 mg/mL
• fluorescence: λ_ex 370 nm; λ_em 420 nm (for cytoplasm of living cells); λ_ex 535 nm; λ_em 610 nm (for chromatin of living cells)
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: −20°C
• SMILES string: CCN1C(c2ccccc2)c3cc(N)ccc3-c4ccc(N)cc14
• InChI: 1S/C21H21N3/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14/h3-13,21H,2,22-23H2,1H3
• InChI key: XYJODUBPWNZLML-UHFFFAOYSA-N

Description

Application

Dihydroethidium has been used as a superoxide probe in bovine pulmonary artery endothelial cell (BPAEC). It has been used to check the in situ generation of ROS (reactive oxygen species).

Redox indicator. Blue fluorescence until oxidized to ethidium.

Packaging

Packaged under Argon.

Linkage

B-ring reduced analog of ethidium

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves