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A New Hyaluronic Acid Derivative Obtained from Atom Transfer Radical Polymerization as a siRNA Vector for CD44 Receptor Tumor Targeting.

A New Hyaluronic Acid Derivative Obtained from Atom Transfer Radical Polymerization as a siRNA Vector for CD44 Receptor Tumor Targeting.

Macromolecular bioscience (2015-07-03)

Fabio Salvatore Palumbo, Antonella Bavuso Volpe, Flavia Bongiovì, Giovanna Pitarresi, Gaetano Giammona

초록

Two derivatives of hyaluronic acid (HA) have been synthesized by atom transfer radical polymerization (ATRP), starting from an ethylenediamino HA derivative (HA-EDA) and by using diethylaminoethyl methacrylate (DEAEMA) as a monomer for polymerization. Both samples, indicated as HA-EDA-pDEAEMA a and b, are able to condense siRNA, as determined by gel retardation assay and resulting complexes show a size and a zeta potential value dependent on polymerization number, as determined by dynamic light scattering measurements. In vitro studies performed on HCT 116 cell line, that over express CD44 receptor, demonstrate a receptor mediated uptake of complexes, regardless of their surface charge.

MATERIALS

제품 번호

브랜드

제품 설명

Sigma-Aldrich

2-Bromo-2-methylpropionic acid, 98%

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Acetone, histological grade, ≥99.5%

Sigma-Aldrich

Acetone, ACS reagent, ≥99.5%

Sigma-Aldrich

Acetone, Laboratory Reagent, ≥99.5%

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Acetone, ACS reagent, ≥99.5%

Sigma-Aldrich

Acetone, puriss., meets analytical specification of Ph. Eur., BP, NF, ≥99% (GC)

Sigma-Aldrich

Acetone, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.5% (GC)

Sigma-Aldrich

Acetone, natural, ≥97%

Sigma-Aldrich

Acetone, ≥99%, meets FCC analytical specifications

Sigma-Aldrich

Tetrabutylammonium hydroxide solution, 1.0 M in methanol

Sigma-Aldrich

Tetrabutylammonium hydroxide solution, 40 wt. % in H₂O

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Tetrabutylammonium hydroxide solution, 53.5-56.5% in H₂O

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Copper(II) bromide, 99%

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Copper(II) bromide, 99.999% trace metals basis

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Diethylamine, puriss. p.a., ≥99.5% (GC)

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Diethylamine, ≥99.5%

Sigma-Aldrich

Dimethyl sulfoxide, Hybri-Max^™, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%

Sigma-Aldrich

1,2-Dichloroethane, ACS reagent, ≥99.0%

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Dimethyl sulfoxide, puriss. p.a., ACS reagent, ≥99.9% (GC)

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N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride, crystalline

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N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride, BioXtra

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N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride, commercial grade, powder

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Deuterium oxide, 99.8 atom % D

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Deuterium oxide, 60 atom % D

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Deuterium oxide, 70 atom % D

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Deuterium oxide, 99.9 atom % D, contains 0.05 wt. % 3-(trimethylsilyl)propionic-2,2,3,3-d₄ acid, sodium salt

Sigma-Aldrich

1,2-Dichloroethane, anhydrous, 99.8%

Sigma-Aldrich

Diethylamine, purified by redistillation, 99.5%

Sigma-Aldrich

N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride, ≥99.0% (AT)

Sigma-Aldrich

Deuterium oxide, 99.9 atom % D, contains 0.75 wt. % 3-(trimethylsilyl)propionic-2,2,3,3-d₄ acid, sodium salt