Electrochemical glucose biosensor based on nickel oxide nanoparticle-modified carbon paste electrode.

Electrochemical glucose biosensor based on nickel oxide nanoparticle-modified carbon paste electrode.

Artificial cells, nanomedicine, and biotechnology (2013-06-26)

Ceren Erdem, Derya Koyuncu Zeybek, Gözde Aydoğdu, Bülent Zeybek, Sule Pekyardımcı, Esma Kılıç

초록

In the present work, we designed an amperometric glucose biosensor based on nickel oxide nanoparticles (NiONPs)-modified carbon paste electrode. The biosensor was prepared by incorporation of glucose oxidase and NiONPs into a carbon paste matrix. It showed good analytical performances such as high sensitivity (367 μA mmolL(-1)) and a wide linear response from 1.9×10(-3) mmolL(-1) to 15.0 mmolL(-1) with a limit of detection (0.11 μmolL(-1)). The biosensor was used for the determination of glucose in human serum samples. The results illustrate that NiONPs have enormous potential in the construction of biosensor for determination of glucose.

MATERIALS

제품 번호

브랜드

제품 설명

08582

Millipore

Urea solution, suitable for microbiology, 40% in H₂O

G7528

Sigma-Aldrich

D-(+)-Glucose, ≥99.5% (GC), BioXtra

47249

Supelco

D-(+)-Glucose, analytical standard

608203

Sigma-Aldrich

D-Glucose-¹²C₆, ¹⁶O₆, 99.9 atom % ¹⁶O, 99.9 atom % ¹²C

47863

Supelco

L-Ascorbic acid, analytical standard

U7129

Sigma-Aldrich

Urea solution, 40 % (w/v) in H₂O

299367

Sigma-Aldrich

Urea-¹²C, 99.9 atom % ¹²C

A4544

Sigma-Aldrich

L-Ascorbic acid, suitable for cell culture, suitable for plant cell culture, ≥98%

A7506

Sigma-Aldrich

L-Ascorbic acid, reagent grade, crystalline

A0278

Sigma-Aldrich

L-Ascorbic acid, reagent grade

255564

Sigma-Aldrich

L-Ascorbic acid, ACS reagent, ≥99%

33034

Sigma-Aldrich

L-Ascorbic acid, puriss. p.a., ACS reagent, reag. ISO, Ph. Eur., 99.7-100.5% (oxidimetric)

95209

Sigma-Aldrich

L-Ascorbic acid, BioUltra, ≥99.5% (RT)

95210

Sigma-Aldrich

L-Ascorbic acid, puriss. p.a., ≥99.0% (RT)

95212

Sigma-Aldrich

L-Ascorbic acid, tested according to Ph. Eur.

W210901

Sigma-Aldrich

L-Ascorbic acid, FCC, FG

57803

Supelco

L-Ascorbic acid, certified reference material, TraceCERT^®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland

A5960

Sigma-Aldrich

L-Ascorbic acid, BioXtra, ≥99.0%, crystalline

A2218

Sigma-Aldrich

L-Ascorbic acid, meets USP testing specifications

A92902

Sigma-Aldrich

L-Ascorbic acid, 99%

G5767

Sigma-Aldrich

D-(+)-Glucose, ACS reagent

49139

Sigma-Aldrich

D-(+)-Glucose, BioUltra, anhydrous, ≥99.5% (sum of enantiomers, HPLC)

G8270

Sigma-Aldrich

D-(+)-Glucose, ≥99.5% (GC)

49152

Sigma-Aldrich

D-(+)-Glucose, tested according to Ph. Eur.

G6152

Sigma-Aldrich

D-(+)-Glucose, suitable for mouse embryo cell culture, ≥99.5% (GC)

G5146

Sigma-Aldrich

D-(+)-Glucose, Hybri-Max^™, powder, BioReagent, suitable for hybridoma

G7021

Sigma-Aldrich

D-(+)-Glucose, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.5%

A4403

Sigma-Aldrich

L-Ascorbic acid, powder, suitable for cell culture, γ-irradiated

04269

Sigma-Aldrich

Sodium phosphate monobasic dihydrate, meets analytical specification of Ph. Eur., BP, USP, FCC, E 339, 98-100.5% (calc. to the dried substance)

71500

Sigma-Aldrich

Sodium phosphate monobasic dihydrate, purum p.a., crystallized, ≥99.0% (T)