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[Determination of beta-galactosidase activity on nitrocellulose plates using 5-bromo-3-indolyl-beta-D-galactopyranoside and tetrazolium salts].

Bioorganicheskaia khimiia (1987-02-01)
A N Markarian, Ia V Voznyฤญ, B B Dzantiev, A M Egorov
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A simple and convenient technique has been developed for detection of beta-galactosidase from E. coli on nitrocellulose sheets using a mixture of 5-bromoindol-3-yl-beta-D-galactopyranoside and nitro blue tetrazolium, which enables rapid detection of fmole (10(-15) mole) quantities of the enzyme at pH 9.5. The technique has the following advantages: the substrates are stable for a long period; reaction products give non-fading intense blue colour, resolution is extremely good with essentially no diffusion.

MATERIALS
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Sigma-Aldrich
Bluo-Gal, reagent for selection of recombinant bacterial clones
Sigma-Aldrich
5-Bromo-3-indolyl ฮฒ-D-galactopyranoside, โ‰ฅ98%