Name: Triton<SUP>™</SUP> X-100
Brand: SIGMA

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About This Item

Linear Formula:

t-Oct-C₆H₄-(OCH₂CH₂)_xOH, x= 9-10

CAS Number:

9036-19-5

Beilstein:

2315025

MDL number:

MFCD00128254

UNSPSC 코드:

12161900

PubChem Substance ID:

329826885

NACRES:

NA.21

기술 서비스

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도움 문의

생물학적 소스

synthetic (organic)

Grade

Molecular Biology

설명

non-ionic

양식

liquid

분자량

micellar avg mol wt 80,000
average mol wt 625

응집 번호

100-155

기술

protein purification: suitable
protein quantification: suitable
western blot: suitable

불순물

<1.00% water (Karl Fischer)

pH

9.7

CMC

0.2-0.9 mM (20-25°C)

mp

6 °C

전이 온도

cloud point 65 °C
pour point ~7 °C

solubility

water: 0.1 mL/mL, clear to slightly hazy, colorless to faintly yellow

density

1.06 g/mL at 25 °C (lit.)

양이온 미량물

Fe: <5 ppm
K: <0.05%
Na: <0.1%
heavy metals: <5 ppm

HLB

13.5

응용 분야

hematology
histology

외래 활성

DNase and RNase, none detected

저장 온도

room temp

SMILES string

CC(C)(C)CC(C)(C)c1ccc(OCCOCCOCCOCCOCCOCCOCCO)cc1

InChI

1S/C28H50O8/c1-27(2,3)24-28(4,5)25-6-8-26(9-7-25)36-23-22-35-21-20-34-19-18-33-17-16-32-15-14-31-13-12-30-11-10-29/h6-9,29H,10-24H2,1-5H3

InChI key

HNLXNOZHXNSSPN-UHFFFAOYSA-N

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일반 설명

Triton^™ X-100 is a common non-ionic surfactant and emulsifier which is often used in biochemical applications to solubilize proteins. It is considered a comparatively mild detergent, non-denaturing, and is reported in numerous references as a routinely added reagent. It is utilized for lysing cells to extract protein and cellular organelles. It can also permeabilize the living cell membrane for transfection.

애플리케이션

Triton^™ X-100 has been used:

In immunohistochemistry for staining the Flat-mount retinas
Along with ice-cold PBS (phosphate buffered saline) in suspension of cells for cell DNA analysis and Annexin V assay
To permeabilise cells during Immunofluorescent microscopic studies
As a positive control in LDH assay to determine the cell membrane integrity
For estimating the lipase activity in postheparin plasma by using modified Belfrage and Vaughan radioenzymatic procedure
For the preparation of outer membrane protein exctract
As a component of extraction buffer along with tris-HCl, NaCl, CaCl₂, ZnCl₂, Brij 35 for homogenization of mice lung cells
In the treatment of tissue sections for Immunofluorescence labeling