S8820
Protease Inhibitor Cocktail
SIGMA FAST, tablet, for the inhibition of serine, cysteine, aspartic and metalloproteases, For General Use
동의어(들):
Protease Inhibitor Tablets, protease inhibitor
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모든 사진(2)
About This Item
UNSPSC 코드:
12352200
NACRES:
NA.77
solubility:
water: 1 tablet/100mL, clear to hazy, colorless
추천 제품
제품명
SIGMAFAST™ Protease Inhibitor Tablets, For General Use
생물학적 소스
bovine lung
bovine milk
Quality Level
양식
tablet
solubility
water: 1 tablet/100mL, clear to hazy, colorless
저장 온도
2-8°C
일반 설명
SIGMAFAST™ Protease Inhibitor Tablets are a water-soluble tablet formulation containing a broad-spectrum cocktail of protease inhibitors, including AEBSF, E-64, bestatin, leupeptin, aprotinin, and EDTA. One tablet can make 100 mL of cocktail and can inhibit proteases present in up to 20 g of cell extract.
The use of SIGMAFAST Protease Inhibitor Tablets is not advised with HIS-Select® products or other immobilized metal affinity chromatography (IMAC) products. These tablets contain EDTA, which is a metal chelator and can potentially remove metal ions from these affinity products.
특이성
Inhibits serine, cysteine, aspartic, and metalloproteases.
애플리케이션
SIGMAFAST Protease Inhibitor Tablets has been used:
- to wash raw semen for membrane protein analysis
- in a solution to incubate CD4+ T cells to obtain total cell lysates for western blot analyses
- as a component of NP-40 buffer to homogenize the spinal cord tissue centered at the injury epicenter to conduct western blot analysis
- as a component in radio-immunoprecipitation assay (RIPA) buffer to homogenize the spinal cord tissue centered at the injury center to detect neuregulin-1 beta 1 (Nrg-1ß1) in spinal cord tissue samples with enzyme-linked immunosorbent assay (ELISA)
These tablets have been optimized and tested for general use. They have been used in various applications, including collecting tracheae in combination with ice-cold radioimmunoprecipitation assay buffer to determine the tracheal basal cell fate, and homogenizing rat lung tissues for western blot analysis.
특징 및 장점
- Broad-spectrum protease inhibition: inhibits serine, cysteine, aspartic, and metalloproteases, making it a versatile solution for protease inhibition in various applications.
- Water-soluble tablet formulation: easy to use and prepare, with one tablet making 100 mL of cocktail.
- Contains a cocktail of protease inhibitors: provides a comprehensive and effective solution for protease inhibition.
- Tested and optimized for general use: proven performance and reliability for a wide range of applications.
성분
AEBSF, 2 mM
Aprotinin, 0.3 μM
Bestatin, 130 μM
EDTA, 1 mM
E-64, 14 μM
Leupeptin, 1 μM
Aprotinin, 0.3 μM
Bestatin, 130 μM
EDTA, 1 mM
E-64, 14 μM
Leupeptin, 1 μM
수량
One tablet makes 100 mL of cocktail. One mL is recommended for the inhibition of proteases equivalent to 1 mg of USP pancreatin. One tablet is recommended for the inhibition of proteases present in a maximum of 20 g of cell extract.
물리적 형태
Tablet that is water soluble.
기타 정보
For R&D use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.
법적 정보
HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany
SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC
관련 제품
제품 번호
설명
가격
신호어
Danger
유해 및 위험 성명서
Hazard Classifications
Eye Dam. 1 - Skin Corr. 1A - STOT SE 3
표적 기관
Respiratory system
Storage Class Code
8A - Combustible corrosive hazardous materials
WGK
WGK 3
개인 보호 장비
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