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Merck
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주요 문서

D9170

Sigma-Aldrich

Dimethyl sulfoxide

PCR Reagent

동의어(들):

DMSO

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About This Item

Linear Formula:
(CH3)2SO
CAS Number:
Molecular Weight:
78.13
Beilstein:
506008
EC Number:
MDL number:
UNSPSC 코드:
12191502
PubChem Substance ID:
NACRES:
NA.25
bp:
189 °C (lit.)
vapor pressure:
0.42 mmHg ( 20 °C)

vapor density

2.7 (vs air)

Quality Level

vapor pressure

0.42 mmHg ( 20 °C)

분석

≥99.5%

양식

liquid

autoignition temp.

573 °F

유통기한

Recommended retest period - 2 years

expl. lim.

42 %, 63 °F

포장

vial of 1 mL

기술

PCR: suitable

불순물

≤0.1% water
water

색상

colorless

refractive index

n20/D 1.479 (lit.)

bp

189 °C (lit.)

mp

16-19 °C (lit.)

solubility

H2O: miscible (completely)

density

1.10 g/mL (lit.)

적합성

suitable for PCR

응용 분야

agriculture

SMILES string

CS(C)=O

InChI

1S/C2H6OS/c1-4(2)3/h1-2H3

InChI key

IAZDPXIOMUYVGZ-UHFFFAOYSA-N

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일반 설명

Dimethyl sulfoxide is a free radical scavenger and a cryopreservant. It elicits protective effect in double stranded breaks(DSBs). Dimethyl sulfoxide (1-10%) has been shown to accelerate strand renaturation and is believed to give the nucleic acid thermal stability against depurination. As a PCR cosolvent, DMSO may help improve yields, especially in long PCR.

애플리케이션

Dimethyl sulfoxide has been used as a component of polymerase chain reaction (PCR) mixture and as PCR facilitator.

주의사항

Supercools easily and remelts slowly at room temperature. Solidified product can be re-liquified by warming to room temperature without detriment to the product.

기타 정보

For information on dimethyl sulfoxide miscibility, please visit the following link:
Dimethyl Sulfoxide Miscibility/Immiscibility Table

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point (°F)

188.6 °F - closed cup

Flash Point (°C)

87 °C - closed cup

개인 보호 장비

Eyeshields, Gloves, type ABEK (EN14387) respirator filter


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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Polymerase chain reaction: basic protocol plus troubleshooting and optimization strategies
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Journal of Visualized Experiments, (63) (2012)
IRAP and REMAP for retrotransposon-based genotyping and fingerprinting
Kalendar R and Schulman AH
Nature Protocols, 1(5), 2478-2478 (2006)
Microtitre Plate Based Cell-SELEX Method
Shorie M and Kaur H
Bio-protocol, 8(20) (2018)
A single-molecule assessment of the protective effect of DMSO against DNA double-strand breaks induced by photo-and gamma-ray-irradiation, and freezing
Noda M, et al.
Scientific reports, 7(1), 8557-8557 (2017)
Development of PCR-Based Technique for Detection of Purity of Pashmina Fiber from Textile Materials
Ravikumar et al.
Applied Biochemistry and Biotechnology, 175(1), 3856-3862 (2015)

프로토콜

Method for bacterial genome analysis and detection of pathogens. Minimize false positive PCRs through lab design and reagents tested for use in bacterial PCR applications.

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