Nucleotides are organic molecules that serve as the monomers, or subunits, of nucleic acids (like DNA and RNA). The building blocks of nucleic acids, nucleotides consist of a nitrogenous base (purine or pyrimidine), a five-carbon sugar (ribose or deoxyribose), and at least one phosphate group. A nucleoside along with a phosphate group yields a nucleotide. The Deoxynucleotide mix is a convenient premixed dNTP solution containing 10 mM each of UltraPure dATP, dCTP, dGTP, and TTP sodium salts in high-quality molecular biology grade water. One µL is sufficient for a standard 50 µL PCR reaction.

dNTP Mix has been used:

• in the PCR amplification of genomic DNA isolated from insect, fungi, virus, human,
• in reverse transcription of total RNA to cDNA.
• as a component of the DNA amplification mixture for polymerase chain reaction (PCR)
• routine and long PCR
• manual and automated DNA sequencing
• cDNA synthesis and labeling reactions