Dulbecco′s Phosphate Buffered Saline is an isotonic saline solution used for washing cells and tissues. This balanced salt solution (BSS) is also used in cell culture as a buffering system to maintain the medium within the physiological pH range and as an irrigating, transporting and diluting fluid while maintaining intra- and extracellular osmotic balance.

Dulbecco′s Phosphate Buffered Saline has been used:

• for decellularization of extracellular matrix (ECM)
• to rinse biosensor chip to remove physically adsorbed mycolic acids
• for washing cells before staining during in vitro biocompatibility test