Name: Ethylenediaminetetraacetic acid
Brand: SIAL

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About This Item

Linear Formula:

(HO₂CCH₂)₂NCH₂CH₂N(CH₂CO₂H)₂

CAS Number:

60-00-4

Molecular Weight:

292.24

Beilstein:

1716295

EC Number:

200-449-4

MDL number:

MFCD00003541

UNSPSC 코드:

12352106

eCl@ss:

39030907

PubChem Substance ID:

24894705

NACRES:

NA.77

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Quality Level

200

Grade

ACS reagent

Agency

suitable for SM 4500 - NH3

분석

99.4-100.6%

양식

powder

반응 적합성

reagent type: chelator

불순물

≤0.005% insoluble matter
≤0.1% Nitrilotriacetic acid ([HOCOCH₂]₃N)

무기 잔류물

≤0.2%

pH

2.5 (23 °C, 10 g/L)

mp

250 °C (dec.) (lit.)

solubility

water: soluble 0.4 g/L at 20 °C

양이온 미량물

Ca: ≤0.001%
Fe: ≤0.005%
Mg: ≤5 ppm
heavy metals: ≤0.001% (byICP-OES)

SMILES string

OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI

1S/C10H16N2O8/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20)

InChI key

KCXVZYZYPLLWCC-UHFFFAOYSA-N

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일반 설명

Ethylenediaminetetraacetic acid (EDTA) is a hydrophilic metal chelating agent that transforms metal ions into inactive, cyclic metal complexes. Hence, it has industrial application for resolving metal contamination.

애플리케이션

Ethylenediaminetetraacetic acid has been used:

as a component of labeling buffer, which is used in the washing step during cell fractionation{155)
for investigating the effect of papain enzyme on wound debridement and other skin issues
for harvesting rat arterial smooth muscle cell after its pre-treatment for its use in flow cytometry analysis

생화학적/생리학적 작용

Ethylenediaminetetraacetic acid (EDTA) is not easily administered by the gastrointestinal tract. Intravenous administration of sodium EDTA results in hypocalcemic tetany. It is generally useful in plasma or molecular diagnostics and acts by chelating calcium. EDTA serves as an anticoagulant for haematological cell counting and cell morphological analysis.