We are committed to bringing you greener alternative products, which adhere to one or more of the 12 Principles of Green Chemistry. This antibody is preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed, and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Clone 1H20-H2 is a ZooMAb^® rabbit recombinant monoclonal antibody that detects IL-1β. It targets an epitope from the C-terminal half.

His-tagged recombinant protein fragment corresponding to 153 amino acids from the C-terminal half of human IL-1β.

Quality Control Testing

Evaluated by Western Blotting in lysate from THP-1 cells treated with Phorbol 12-myristate 13-acetate (PMA)/Lipopolysaccharide.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected IL-1β in lysate from THP-1 cells treated with Phorbol 12-myristate 13-acetate (PMA; 80 nM; 24 h) and Lipopolysaccharide (LPS; 1 g/mL; 15 min).

Tested Applications

Affinity Binding Assay: A representative lot of this antibody bound IL-1β protein with at least one thousand-fold (1,000X) higher affinity than with non-specific GST control.

Immunocytochemistry Analysis: 1:100 dilution from a representative lot detected IL-1β in THP-1 cells treated with phorbol 12-myristate 13-acetate (PMA; 80 nM; 24 h) then Lipopolysaccharides (LPS; 1

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Interleukin-1 beta (UniProt: P01584; also known as IL-1 beta, Catabolin) is encoded by the IL1B (also known as IL1F2) gene (Gene ID: 3553) in human. IL-1β is one of the best characterized potent pro-inflammatory cytokines that is critical for host-defense responses to infection and injury. It is produced and secreted by a variety of cell types; however, it is mainly expressed in activated monocytes/macrophages. IL-1β is produced as pro-IL-1β in response to molecular motifs carried by pathogens known as pathogen associated molecular patterns (PAMPs). Its synthesis occurs in two steps and each step is controlled by different stimuli. In the initial step, inflammatory signals, such as lipopolysaccharides, stimulate the synthesis and promote the accumulation of pro-IL-1β in cytoplasm. Following this step pro-IL-1β is cleaved by the caspase-1, the activation of which occurs via recruitment to inflammasome. This step removes the propeptide (1-116) segment. Following caspase-1-dependent processing of pro-IL-1β, mature IL-1β (aa 117-269) is rapidly secreted from cells. IL-1β is known to induce prostaglandin synthesis, neutrophil influx and activation, T-cell and B-cell activation and antibody production. It also promotes Th17 differentiation and synergizes with IL-12 to induce interferon g from T-helper cells. This ZooMAb^® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Lopez-Castejon, G., and Brough, D. (2011). Cytokine Growth Factor Rev. 22(4); 189-195; Tominaga, K., et al. (2000). Int. Immunol. 12(2); 151-160).

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.