Specific for human prion protein (PrP). This antibody immunolabels amyloid plaques in formalin-fixed paraffin sections from Creutzfeld-Jakob Disease (CJD) brain.The prion protein is a large membrane protein that occurs normally in neurons of the human brain and is thought to be involved in synaptic transmission. In prion diseases, such as CJD, Gerstmann-Straussler-Scheinker syndrome (GSS), Fatal Familial Insomnia (FFI), Alpers Syndrome and Kuru, the normal cellular form of this protein (PrPc) is transformed into an altered protein when it comes into contact with an infectious prion protein (PrPsc) from another host. This altered PrPsc accumulates in cytoplasmic vesicles of diseased individuals forming lesions, vacuoles and amyloid deposits.

Epitope: N-terminus, a.a. 78-97

Synthetic peptide that corresponds to amino acids 79-97 of the N-terminus of the human PrP27-30.

Anti-Prion Protein Antibody, N-terminus, a.a. 78-97 detects level of Prion Protein & has been published & validated for use in ELISA, WB, IH(P).

Immunohistochemistry: >1:200 on paraffin embedded, formalin fixed human brain.

Western blots: >1:2,000

ELISA: >1:35,000

Optimal working dilutions must be determined by the end user.

Research Category
Neuroscience

Research Sub Category
Neurodegenerative Diseases

Goat serum. Liquid containing 0.01% thimerosal

Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.

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